Abstract
Stress ulcer occurs primarily in severe conditions, with a high incidence and mortality in intensive care units. However, studies on the association between stress ulcer and bile reflux to the stomach with stress ulcer are still inconclusive. Therefore, our research aimed to determine whether or not bile reflux exists during stress ulcer and then to investigate the effects and mechanism of changes of pyloric local neurotransmitters on bile reflux in such circumstances so as to provide a new pathway for clinical intervention. Cold water immersion was used to copy the stress ulcer model of rats. Sixty-five adult Sprague–Dawley rats of either sex were randomly divided into three groups: the normal control group (n = 10), the stress group (n = 30), and the antagonist group (n = 25). The gastric ulcer index, pH, and bile acid of gastric juice were measured before and after stress. Radio Immunoassay Detection Kit and Biochemic Detection Kit were used to measure local contents of CGRP (calcitonin gene-related peptide) and nitric oxide, respectively, in rats' pylorus. The local contents of nitric oxide in rats' pylorus reached a maximum at 1 hr after stress. The bile acid and pH of gastric juice peaked at 2 hr after stress and the ulcer index peaked at 4 hr after stress. But the local contents of CGRP in rats' pylorus decreased to the minimum at 4 hr after stress. The bile acid and ulcer index in the L-NAME group were significantly lower than in the antagonist control group. However, the bile acid in the hCGRP8-37 group was less than in the antagonist control group. Compared with hCGRP8-37 group, there was a significant reduction in bile acid in the L-NAME group. There was a significant reduction in the ulcer index of the hCGRP8-37 group compared with the L-NAME group and the antagonist control group. There was a certain kind of positive correlation between nitric oxide in rats' pylorus and bile acid to the stomach, for nitric oxide could loosen the pyloric sphincter and increase the bile acid to the stomach. L-NAME might reduce the local nitric oxide contents in rats' pylorus so that bile acid to the stomach might be decreased, obviously with a looser tight pyloric sphincter. Meanwhile, the CGRP in rats' pylorus was negatively associated with the ulcer index, hence CGRP might protect gastric mucosa under stress conditions.
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References
Daley RJ, Rebuck JA, Welage LS, Rogers FB: Prevention of stress ulceration: current trends in critical care. Crit Care Med 32(10):2008–2013, 2004
Fukuhara K, Osugi H, Takada N, Takemura M, Lee S, Taguchi S, Kaneko M, Tanaka Y, Fujiwara Y, Nishizawa S, Kinoshita H: Correlation between duodenogastric reflux and remnant gastritis after distal gastrectomy. Hepatogastroenterology 51:1241–1244, 2004
Li X, Yu AG, Deng ZP, Liu YS, Li YJ, Yang CH, Lin JT, Hu YZ: Study on pathogenesis and prevention of stress ulcer of rats. Chin Basic Clin J Gen Surg 7:138–140, 2000
Zhu AY, Xu GM, Li ZS, Yin N, Zou DW: Monitoring of bile reflux for patients with chronic gastritis. Chin J Dig 23:530–531, 2002
Tan DY, Yao HC: Monitoring of bile reflux for patients with functional dyspepsia and gastric motility. Chin J Dig 32:313–314, 2002
Qui BS, Mei QB, Liu L, Tchou-Wong KM: Effects of nitric oxide on gastric ulceration induced by nicotine and cold-restraint stress. W J Gastroenterol 10:594–597, 2004
Chen SZ, Zhao H, Wu CY, Fu WH, Chen XC: Gallbladder emptying function in patients with bile reflux gastritis. World Chin J Gastroenterol 6:427–429, 1998
Zhang J, Li DZ, Hu HB, Wen XD, Wu QP: Experimental and clinical research about the treatment of Talcid on bile reflux gastritis. Chin J Clin Med 11:75–77, 2002
Cui ZM, Li ZS, Xu GM, Zhan XB: Influence of L-NAME and L-arg on gastric mucosal tolerant cytoprotection under stress. Chin J Dig 11:219–222, 2002
Bayguinov O, Sanders KM: Role of nitric oxide as an inhibitory neurotransmitter in the canine pyloric sphincter. Am J Physiol 264(5, Pt 1):G975–G983, 1993
Zhang LD, Gao GH, Su X, Jin ZJ, Bao J: Possible relationship between protective effect of calcitonin gene-related peptide on gastric stress ulcer and nitric oxide content in stomach. J Clin Paediatr 19:308–309, 2001
Zhang LD, Su X, Jin ZJ, Bao J, Xu T: The protective effect of calcitonin gene-related peptide on gastric stress ulcer of young rats. Chin J Dig 21:187–188, 2001
Dong XY, Wang LX, Zhou LY, Lin SR: The effect and their mechanism of gastric mucosal drugs. Chin J Dig 22:526–529, 2002
Wang X, Zhong YX, Zhang ZY, Lu J, Lan M, Miao JY, Guo XG, Shi YQ, Zhao YQ, Ding J, Wu KC, Pan BR, Fan DM: Effect of L-NAME on nitric oxide and gastrointestinal motility alterations in cirrhotic rats. W J Gastroenterol 8:328–332, 2002
Qu SY, Li W, Zheng TZ: The role of nitric oxide in gastrointestinal motility and pathogenesis of digestive diseases. Rec Dev Physiol 26:77–79, 1995
Fan YP, Chakder S, Gao F, Rattan S: Inducible and neuronal nitric oxide synthase involvement in lipopolysaccharide-induced sphincteric dysfunction. Am J Physiol Gastrointest Liver Physiol 280:32–42, 2001
Mashimo H, Kjellin A, Goyal RK: Gastric stasis in neuronal nitric oxide synthase-deficient knockout mice. Gastroenterology 119:766–773, 2001
Hang CH, Shi JX, Li JS, Wu W, Li WQ, Yin HX: Levels of vasoactive intestinal peptide, cholecycstokinin and calcitonin gene-related peptide in plasma and jejunum of rats following traumatic brain injury and underlying significance in gastrointestinal dysfunction. W J Gastroenterol 10:875–880, 2004
Grisoni E, Dusleag D, Super D: Nitric oxide synthesis inhibition: the effect on rabbit pyloric muscle. J Pediatr Surg 31(6):800–804, 1996
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Min, C., Hesheng, L., Jihong, C. et al. Effects and Mechanism of Changes of Local Neurotransmitters in Rats' Pylorus and Bile Reflux to the Stomach with Stress Ulcer. Dig Dis Sci 50, 1898–1903 (2005). https://doi.org/10.1007/s10620-005-2958-1
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DOI: https://doi.org/10.1007/s10620-005-2958-1