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Downregulation of Cytokine Release by Heat Preconditioning of Livers Used for Transplantation in Rats

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Abstract

We evaluated the changes in the levels of released cytokines following heat preconditioning of the livers used in rat liver transplantation. The donor rats in the heat preconditioning (HP) group were subjected to heat preconditioning 48 h before graft harvesting. The liver isografts were preserved in Euro-Collins solution for 8 h, and then transplanted orthotopically. The one-week survival rate of the HP group was significantly better than that of the control (C) group. The serum levels of interleukin-6 and interleukin-10 were significantly lower in the HP group than in the C group. Histological staining revealed that the stagnation of red blood cells and infiltration of neutrocytes were reduced in the HP group. The expression of intercellular adhesion molecule-1 was decreased around the central vein in the HP group, as revealed by immunohistochemistry. These results indicate that heat preconditioning downregulates cytokine release and reduces the frequency of microcirculation disorders.

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Authors and Affiliations

  1. First Departments of Surgery, Ehime University, Ehime, Japan

    Jota Watanabe MD, Fumiki Kushihata MD, Kinya Matsumoto MD, Kazuo Honda MD & Nobuaki Kobayashi MD

  2. Departments of Anatomy, Ehime University, Ehime, Japan

    Seiji Matsuda MD

  3. First Department of Surgery, School of Medicine, Ehime University, Shitsukawa Toon, Ehime, 791-0295, Japan

    Jota Watanabe MD

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  1. Jota Watanabe MD
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  2. Fumiki Kushihata MD
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  3. Kinya Matsumoto MD
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  4. Kazuo Honda MD
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  5. Seiji Matsuda MD
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  6. Nobuaki Kobayashi MD
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Correspondence to Jota Watanabe MD.

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Watanabe, J., Kushihata, F., Matsumoto, K. et al. Downregulation of Cytokine Release by Heat Preconditioning of Livers Used for Transplantation in Rats. Dig Dis Sci 50, 1823–1828 (2005). https://doi.org/10.1007/s10620-005-2945-6

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  • DOI: https://doi.org/10.1007/s10620-005-2945-6

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