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Effect of Bacterial Chemotactic Peptides on Intestinal Inflammation in Animal Models of Acute and Chronic “Relapsed” Colitis

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Abstract

It is known that bacterial chemotactic peptides such as formyl-methionyl-leucyl-phenylalanine (fMLP) exacerbate colitis during the acute phase, but the precise role of fMLP during chronic “relapse” is unknown. In this study we examined the effect of bacterial peptides in animal models of acute and chronic “relapsed” colitis. Different parameters were evaluated, such as tissue damage, myeloperoxidase activity, and mucosal function. In acute trinitrobenezene sulfonic acid colitis, fMLP had significant adverse effects on mucosal function and worsened several parameters. In contrast, in chronic “relapsed” colitis the ability of fMLP to exacerbate the inflammation was dependent on whether it was confined to the lumen of the colon. Bacterial peptides such as fMLP appear to play a different role in the acute phase of inflammation compared with the chronic phase, depending on the integrity of the mucosal barrier.

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Authors and Affiliations

  1. Department of Physiology, Ponce School of Medicine, P.O. Box 7004, Ponce, Puerto Rico, 00732-7004

    Gerardo A. HernÁndez PhD, Melanie R. ValentÍn BS & Caroline B. Appleyard PhD

Authors
  1. Gerardo A. HernÁndez PhD
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  2. Melanie R. ValentÍn BS
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  3. Caroline B. Appleyard PhD
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Corresponding author

Correspondence to Caroline B. Appleyard PhD.

Additional information

The experiments reported herein were performed in accordance with the principles described in the “Guide for the Care and Use of Laboratory Animals,” Publication No. DHHS (NIH) 86-23.

This work was supported by National Institutes of Health grants including National Research Service Award F31DK60245 from the NIDDK (G.A.H.), S06-GM08239 (C.B.A.), and G12-RR03050 (C.B.A.).

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HernÁndez, G.A., ValentÍn, M.R. & Appleyard, C.B. Effect of Bacterial Chemotactic Peptides on Intestinal Inflammation in Animal Models of Acute and Chronic “Relapsed” Colitis. Dig Dis Sci 50, 1444–1453 (2005). https://doi.org/10.1007/s10620-005-2859-3

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  • DOI: https://doi.org/10.1007/s10620-005-2859-3

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