Digestive Diseases and Sciences

, Volume 49, Issue 11–12, pp 1752–1757 | Cite as

Alterations in Antioxidant Power and Levels of Epidermal Growth Factor and Nitric Oxide in Saliva of Patients with Inflammatory Bowel Diseases

  • Golshid Jahanshahi
  • Vian Motavasel
  • Ali Rezaie
  • Ali A. Hashtroudi
  • Naser E. Daryani
  • Mohammad AbdollahiEmail author


Despite extensive investigation, the pathophysiology of human inflammatory bowel disease (IBD) remains incompletely understood. We examined the existence of oxidative and nitrosative stress and also alterations in epidermal growth factor (EGF) secretion in saliva of IBD patients. Saliva samples were obtained from 30 nonsmoking IBD patients including 16 Crohn’s disease (CD) patients and 16 ulcerative colitis (UC) patients and 16 age- and sex-matched controls. Samples were analyzed for thiobarbituric reactive substances (TBARS) as a marker of lipid peroxidation, ferric reducing ability (antioxidant power), and EGF and nitric oxide (NO) levels. Saliva TBARS levels increased significantly (P &< 0.01) in CD patients but not in UC patients. Analysis of antioxidant power revealed that saliva of CD patients has lower antioxidant power (P &< 0.01) than saliva of the healthy control population. The concentration of EGF was found to be increased (P &< 0.01) in saliva of CD patients in comparison to that of healthy subjects. NO levels increased in saliva of both CD and UC patients in comparison to that of healthy subjects. It is concluded that excessive NO production is present in saliva of both CD and UC patients but only saliva of CD patients is oxidatively stressed. EGF secretion is normal in UC patients, although CD patients show a significant increase in salivary EGF levels.

Crohn’s disease ulcerative colitis inflammatory bowel disease oxidative stress nitric oxide epidermal growth factor saliva-5 


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Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • Golshid Jahanshahi
    • 1
  • Vian Motavasel
    • 1
  • Ali Rezaie
    • 1
  • Ali A. Hashtroudi
    • 2
  • Naser E. Daryani
    • 2
  • Mohammad Abdollahi
    • 1
    Email author
  1. 1.Faculty of Pharmacy and Pharmaceutical Sciences Research CenterTehran University of Medical SciencesIran
  2. 2.Department of Gastrointestinal DiseaseTehran University of Medical SciencesIran

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