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MicroRNA-106b-5p (miR-106b-5p) suppresses the proliferation and metastasis of cervical cancer cells via down-regulating fibroblast growth factor 4 (FGF4) expression


This study aims to investigate the function and mechanism of microRNA-106b-5p (miR-106b-5p) in cervical cancer (CC). Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed to determine miR-106b-5p expression in CC tissues and normal gastric tissues. Cell counting kit-8 (CCK-8) and colony formation assays were used to analyze the regulatory effects of miR-106b-5p on CC cells’ proliferative ability. Wound healing and Transwell assays were conducted to detect the effects of miR-106b-5p on cell migration and invasion. Besides, TargetScan was used to predict the potential target genes of miR-106b-5p. The interaction between miR-106b-5p and fibroblast growth factor 4 (FGF4) was proved by qRT-PCR, Western blot, and dual-luciferase reporter gene assay. MiR-106b-5p expression was down-regulated in CC tissues compared to non-tumorous tissues. The expression of miR-106b-5p was associated with the lymphatic node metastasis, FIGO stage and differentiation of CC. Functional assays revealed that miR-106b-5p overexpression suppressed CC cell proliferation, migration and invasion while miR-106b-5p inhibitor had the opposite effects. In addition, FGF4 was identified as a target gene of miR-106b-5p, and FGF could be negatively regulated by miR-106b-5p. MiR-106b-5p may serve as a tumor suppressor in CC, which can inhibit CC growth and metastasis by down-regulating FGF4 expression.

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Data availability

The data used to support the findings of this study are available from the corresponding author upon request.



3′ Untranslated regions


Cervical cancer


Cell counting kit-8


Fibroblast growth factor 4


Fibroblast growth factors






Quantitative reverse transcription-polymerase chain reaction


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We thank Hubei Yican Health Industry Co., Ltd. (Wuhan, China) for its linguistic assistance during the preparation of this manuscript.


This study is supported by the Natural Science Foundation of Dalian Medical University.

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Authors and Affiliations



Conceived and designed the experiments: XXY and FZJ; Performed the experiments: LHW, LJ and XXY; Analyzed the data: LHW and LJ; Wrote the paper: LHW and LJ.

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Correspondence to Xu Xiaoying or Fan Zhijun.

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The authors declare that they have no competing interests.

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Our study was approved by the ethics review board of the Second Hospital of Dalian Medical University.

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Hongwei, L., Juan, L., Xiaoying, X. et al. MicroRNA-106b-5p (miR-106b-5p) suppresses the proliferation and metastasis of cervical cancer cells via down-regulating fibroblast growth factor 4 (FGF4) expression. Cytotechnology (2022).

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  • miR-106b-5p
  • FGF4
  • Cervical cancer
  • Proliferation
  • Metastasis