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Static magnetic field regulates proliferation, migration, and differentiation of human dental pulp stem cells by MAPK pathway

Abstract

Magnetic materials are now commonly used in dental clinics. These materials generally produce a static magnetic field (SMF). While it is known that SMF can affect cells’ behaviors such as proliferation, migration, and differentiation, the mechanisms underlying these effects are still unclear. Our study investigates the role of the mitogen-activated protein (MAP) kinase pathway in SMF-induced proliferation, migration, osteogenic/odontogenic differentiation, and mineralization in human dental pulp stem cells (DPSCs). Human DPSCs were exposed to SMF of 1 mT and the phosphorylated MAP kinases were detected by Western blot analysis. Three MAP kinases inhibitors were pre-cultured with DPSCs and exposed to SMF for 24 h. Cell viability was analyzed using Cell Counting Kit-8. Cell migration was tested by a wound healing assay. Osteogenic/odontogenic differentiation was detected by ALP staining assay, ALP and DSPP Western blot analysis. Mineralization was studied by alizarin red staining analysis. SMF activated phosphorylation of c-Jun N-terminal kinase (JNK), P38 and extracellular signal-regulated kinase (ERK). The inhibition of JNK, P38, and ERK signaling decreased SMF-induced proliferation and migration. ERK and P38 play more important roles in SMF-induced ALP staining and protein expression. JNK was vital for SMF-induced DSPP expression. JNK, P38, and ERK all involved in SMF-mediated mineralization. Our study demonstrated that the MAPK pathway regulated SMF-induced proliferation, migration, osteogenic/odontogenic differentiation, and mineralization in human DPSCs.

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All data generated or analysed during this study are included in this published article.

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Acknowledgements

This work was supported by National Natural Science Foundation of China [grant numbers 11972067, 32171310, 11827803, 11421202, U20A20390], the Fundamental Research Funds for the Central Universities, the 111 Project [grant numbers B13003].

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Correspondence to Lisha Zheng or Yubo Fan.

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The authors deny any conflicts of interest related to this study.

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This study conforms to the Helsinki Declaration, and all processes were approved by the Beihang University Ethical Committee.

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Na, J., Zhang, L., Zheng, L. et al. Static magnetic field regulates proliferation, migration, and differentiation of human dental pulp stem cells by MAPK pathway. Cytotechnology 74, 395–405 (2022). https://doi.org/10.1007/s10616-022-00533-3

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  • DOI: https://doi.org/10.1007/s10616-022-00533-3

Keywords

  • Dental pulp stem cells
  • Osteogenic differentiation
  • Odontogenic differentiation
  • Static magnetic field
  • MAPK