Static magnetic field regulates proliferation, migration, and differentiation of human dental pulp stem cells by MAPK pathway

Na, Jing; Zhang, Lingyu; Zheng, Lisha; Jiang, Jingyi; Shi, Qiusheng; Li, Chiyu; Fan, Yubo

doi:10.1007/s10616-022-00533-3

Original Article
Published: 20 April 2022

Static magnetic field regulates proliferation, migration, and differentiation of human dental pulp stem cells by MAPK pathway

Jing Na¹^na1,
Lingyu Zhang¹^na1,
Lisha Zheng ORCID: orcid.org/0000-0001-9344-2964¹,
Jingyi Jiang¹,
Qiusheng Shi¹,
Chiyu Li¹ &
Yubo Fan¹

Cytotechnology volume 74, pages 395–405 (2022)Cite this article

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Abstract

Magnetic materials are now commonly used in dental clinics. These materials generally produce a static magnetic field (SMF). While it is known that SMF can affect cells’ behaviors such as proliferation, migration, and differentiation, the mechanisms underlying these effects are still unclear. Our study investigates the role of the mitogen-activated protein (MAP) kinase pathway in SMF-induced proliferation, migration, osteogenic/odontogenic differentiation, and mineralization in human dental pulp stem cells (DPSCs). Human DPSCs were exposed to SMF of 1 mT and the phosphorylated MAP kinases were detected by Western blot analysis. Three MAP kinases inhibitors were pre-cultured with DPSCs and exposed to SMF for 24 h. Cell viability was analyzed using Cell Counting Kit-8. Cell migration was tested by a wound healing assay. Osteogenic/odontogenic differentiation was detected by ALP staining assay, ALP and DSPP Western blot analysis. Mineralization was studied by alizarin red staining analysis. SMF activated phosphorylation of c-Jun N-terminal kinase (JNK), P38 and extracellular signal-regulated kinase (ERK). The inhibition of JNK, P38, and ERK signaling decreased SMF-induced proliferation and migration. ERK and P38 play more important roles in SMF-induced ALP staining and protein expression. JNK was vital for SMF-induced DSPP expression. JNK, P38, and ERK all involved in SMF-mediated mineralization. Our study demonstrated that the MAPK pathway regulated SMF-induced proliferation, migration, osteogenic/odontogenic differentiation, and mineralization in human DPSCs.

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Acknowledgements

This work was supported by National Natural Science Foundation of China [grant numbers 11972067, 32171310, 11827803, 11421202, U20A20390], the Fundamental Research Funds for the Central Universities, the 111 Project [grant numbers B13003].

Author information

Jing Na and Lingyu Zhang, both contributed equally to this work.

Authors and Affiliations

Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Xue Yuan Road No.37, Haidian District, Beijing, 100083, China
Jing Na, Lingyu Zhang, Lisha Zheng, Jingyi Jiang, Qiusheng Shi, Chiyu Li & Yubo Fan

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Jing Na
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Lingyu Zhang
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Lisha Zheng
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Jingyi Jiang
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Qiusheng Shi
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Chiyu Li
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Yubo Fan
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Correspondence to Lisha Zheng or Yubo Fan.

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This study conforms to the Helsinki Declaration, and all processes were approved by the Beihang University Ethical Committee.

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Na, J., Zhang, L., Zheng, L. et al. Static magnetic field regulates proliferation, migration, and differentiation of human dental pulp stem cells by MAPK pathway. Cytotechnology 74, 395–405 (2022). https://doi.org/10.1007/s10616-022-00533-3

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Received: 21 October 2021
Accepted: 29 March 2022
Published: 20 April 2022
Issue Date: June 2022
DOI: https://doi.org/10.1007/s10616-022-00533-3

Keywords

Dental pulp stem cells
Osteogenic differentiation
Odontogenic differentiation
Static magnetic field
MAPK