Skip to main content
SpringerLink
Log in

Preparation of CoS nanoparticles-cisplatin bio-conjugates and investigation of their effects on SH-SY5Y neuroblastoma cell line

  • Original Article
  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

Neuroblastoma is one of the most widely seen under the age of 15 tumors that occur in the adrenal medulla and sympathetic ganglia. Cisplatin, an antineoplastic drug, is a Platinum-based compound and is known to inhibit the proliferation of neuroblastoma cells. Effective applications of nanoparticles in biomedical areas such as biomolecular, antimicrobial detection and diagnosis, tissue engineering, theranostics, biomarking, drug delivery, and anti-cancer have been investigated in many studies. This study aims to prepare the bioconjugates of CoS (cobalt sulfide) nanoparticles (NPs) with cisplatin combination groups and to evaluate their effects on the neuroblastoma cell line. Nanoparticle synthesis was done using the green synthesis technique using Punica granatum plant extract. The size and shape of CoS NPs were characterized by SEM, FT-IR, and XRD. Zeta potential was confirmed by the DLS study. For this purpose, the SH-SY5Y neuroblastoma cell line was cultured in a suitable cell culture medium. Cisplatin 5 µg and different concentrations (Cisplatin + CoS NPs bioconjugates (5, 10, 25, 50, 75 μg) doses were applied to SH-SY5Y neuroblastoma cell lines for 24 h. TAC, TOS and MTT tests were performed 24 h after the application. According to the MTT test results, cisplatin and CoS NP combinations reduced the proliferation of neuroblastoma cells by 78 to 57% compared to the cisplatin control. From the findings obtained; the most effective Bio-conjugate group was Cisplatin 5 μg/mL + CoS 75 μg/mL.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • Abudayyak M, Gurkaynak TA, Ozhan G (2017) In vitro evaluation of cobalt oxide nanoparticle-induced toxicity. Toxicol Ind Health 33:646–654

    Article  CAS  Google Scholar 

  • Akhtar MJ, Ahamed M, Alhadlaq HA et al (2017) Nanotoxicity of cobalt induced by oxidant generation and glutathione depletion in MCF-7 cells. Toxicol In Vitro 40:94–101

    Article  CAS  Google Scholar 

  • Alarifi S, Ali D et al (2013) Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells. Int J Nanomedicine 8:189–199

    PubMed  PubMed Central  Google Scholar 

  • Alavi MA, Morsali A (2010) Syntheses and characterization of Mg(OH)(2) and MgO nanostructures by ultrasonic method. Ultrason Sonochem 17:441–446

    Article  CAS  Google Scholar 

  • Ali ZA, Roslan MA, Yahya R et al (2017) Eco-friendly synthesis of silver nanoparticles and its larvicidal property against fourth instar larvae of Aedes aegypti. IET Nanobiotechnol 11:152–156

    Article  Google Scholar 

  • Al-Majed AA (2007) Carnitine deficiency provokes cisplatin-induced hepatotoxicity in rats. Basic Clin Pharmacol Toxicol 100:145–150

    Article  CAS  Google Scholar 

  • Apsana G, George PP, Devanna N (2018) Eco-friendly approach for the efficient biosynthesis of Mg-3(PO4)(2) nanoparticles using plant extract of Ocimum sanctum. Adv Sci Lett 24:5514–5518

    Article  Google Scholar 

  • Balayeva OO, Azizov AA, Muradov MB et al (2017) Synthesis and physicochemical characterization of CuxS-NiySz/FNBR and CuS-NiySz/FNBR nanocomposites from beta-NiS/FNBR by ion exchange method: (x=1; 1.8); (y=1; 3); (z=1; 4). Mater Chem Phys 201:42–49

    Article  CAS  Google Scholar 

  • Basu A, Krishnamurthy S (2010) Cellular responses to Cisplatin-induced DNA damage. J Nucleic Acids. https://doi.org/10.4061/2010/201367

    Article  PubMed  PubMed Central  Google Scholar 

  • Bejarbaneh M, Moradi-Shoeili Z, Jalali A et al (2020) Synthesis of cobalt hydroxide nano-flakes functionalized with glutamic acid and conjugated with thiosemicarbazide for anticancer activities against human breast cancer cells. Biol Trace Elem Res. https://doi.org/10.1007/s12011-020-02049-3 (Epub ahead of print 2020/01/27)

    Article  PubMed  Google Scholar 

  • Chattopadhyay S, Dash SK, Tripathy S et al (2015) Cobalt oxide nanoparticles induced oxidative stress linked to activation of TNF-alpha/caspase-8/p38-MAPK signaling in human leukemia cells. J Appl Toxicol 35:603–613

    Article  CAS  Google Scholar 

  • Dasari S, Tchounwou PB (2014) Cisplatin in cancer therapy: molecular mechanisms of action. Eur J Pharmacol 740:364–378

    Article  CAS  Google Scholar 

  • Donzelli E, Carfi M, Miloso M et al (2004) Neurotoxicity of platinum compounds: comparison of the effects of cisplatin and oxaliplatin on the human neuroblastoma cell line SH-SY5Y. J Neurooncol 67:65–73

    Article  Google Scholar 

  • Groh T, Hrabeta J, Khalil MA et al (2015) The synergistic effects of DNA-damaging drugs cisplatin and etoposide with a histone deacetylase inhibitor valproate in high-risk neuroblastoma cells. Int J Oncol 47:343–352

    Article  CAS  Google Scholar 

  • Gungor AA, Nadaroglu H, Gultekin DD (2019) Synthesis and characterization of nano-strontium oxide (SrO) Using erzincan cimin grape (Vitis vinifera, Cimin). Chem Sci Int J. https://doi.org/10.9734/csji/2019/v26i330092.1-7

    Article  Google Scholar 

  • Hartmann JT, Lipp HP (2003) Toxicity of platinum compounds. Expert Opin Pharmacother 4:889–901

    Article  CAS  Google Scholar 

  • Hartmann JT, Fels LM, Knop S et al (2000) A randomized trial comparing the nephrotoxicity of cisplatin/ifosfamide-based combination chemotherapy with or without amifostine in patients with solid tumors. Invest New Drugs 18:281–289

    Article  CAS  Google Scholar 

  • Hayat MM, Sohail M, Ashraf M (2019) Spectrophotometric determination of cisplatin, carboplatin and oxaliplatin in pure and injectable dosage forms. Biomed Res 30:557–562

    Article  CAS  Google Scholar 

  • Karmous I, Pandey A, Ben Haj K et al (2020) Efficiency of the green synthesized nanoparticles as new tools in cancer therapy: insights on plant-based bioengineered nanoparticles, biophysical properties, and anticancer roles. Biol Trace Elem Res 196:330–342

    Article  CAS  Google Scholar 

  • Kristl M, Dojer B, Gyergyek S et al (2017) Synthesis of nickel and cobalt sulfide nanoparticles using a low cost sonochemical method. Heliyon. https://doi.org/10.1016/j.heliyon.2017.e00273

    Article  PubMed  PubMed Central  Google Scholar 

  • Lojk J, Repas J, Veranic P et al (2020) Toxicity mechanisms of selected engineered nanoparticles on human neural cells in vitro. Toxicology 432:152364

    Article  CAS  Google Scholar 

  • Muradov MB, Balayeva OO, Azizov AA et al (2018) Synthesis and characterization of cobalt sulfide nanoparticles by sonochemical method. Infrared Phys Technol 89:255–262

    Article  CAS  Google Scholar 

  • Nadaroglu H, Azize SI, Gungor AA (2017) Green synthesis of gold nanoparticles using quail egg yolk and investigation of potential application areas. Green Process Synth 6:43–48

    CAS  Google Scholar 

  • Nadaroglu H, Gungor AA, Ince S et al (2017) Green synthesis and characterisation of platinum nanoparticles using quail egg yolk. Spectrochim Acta A Mol Biomol Spectrosc 172:43–47

    Article  CAS  Google Scholar 

  • Nadaroglu H, Onem H, Gungor AA (2017) Green synthesis of Ce2O3 NPs and determination of its antioxidant activity. IET Nanobiotechnol 11:411–419

    Article  Google Scholar 

  • Ratner N, Brodeur GM, Dale RC et al (2016) The “Neuro” of neuroblastoma: neuroblastoma as a neurodevelopmental disorder. Ann Neurol 80:13–23

    Article  Google Scholar 

  • Saad SY, Najjar TAO, Alashari M (2004) Role of non-selective adenosine receptor blockade and phosphodiesterase inhibition in cisplatin-induced nephrogonadal toxicity in rats. Clin Exp Pharmacol Physiol 31:862–867

    Article  CAS  Google Scholar 

  • Sayir F, Sehitogullan A, Demir H et al (2019) Serum prolidase activity, total oxidant/antioxidant, and nitric oxide levels in patients with esophageal squamous cell carcinoma. Turk Gogus Kalp Damar Cerrahisi Derg 27:206–211

    Article  Google Scholar 

  • Taniguchi N (1989) Glutathione centennial: molecular perspectives and clinical implications. Academic Press, Elsevier

    Google Scholar 

  • Wei M, Yuan XJ (2019) Cisplatin-induced ototoxicity in children with solid tumor. J Pediatr Hematol Oncol 41:E97–E100

    Article  CAS  Google Scholar 

  • Xicoy H, Wieringa B, Martens GJ (2017) The SH-SY5Y cell line in Parkinson’s disease research: a systematic review. Mol Neurodegener 12:10

    Article  Google Scholar 

  • Yousef MI, Saad AA, El-Shennawy LK (2009) Protective effect of grape seed proanthocyanidin extract against oxidative stress induced by cisplatin in rats. Food Chem Toxicol 47:1176–1183

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology, Faculty of Pharmacy, Agri Ibrahim Cecen University, Agri, Turkey

    Muhammed Sait Ertugrul

  2. Department of Food Technology, Vocational College of Technical Science, Ataturk University, 25240, Erzurum, Turkey

    Hayrunnisa Nadaroglu

  3. Department of Nano-Science and Nano-Engineering, Institute of Science and Technology, Ataturk University, 25240, Erzurum, Turkey

    Hayrunnisa Nadaroglu

  4. Department of Medical Pharmacology, Faculty of Medicine, Ondokuz Mayıs University, Samsun, Turkey

    Ozge Balpinar Nalci

  5. Department of Medical Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey

    Ahmet Hacimuftuoglu

  6. Department of Nursing, Faculty of Health Sciences, Sakarya University of Applied Sciences, 54187, Sakarya, Turkey

    Azize Alayli

Authors
  1. Muhammed Sait Ertugrul
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hayrunnisa Nadaroglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ozge Balpinar Nalci
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ahmet Hacimuftuoglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Azize Alayli
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

HN designed research; AA, MSE, and OBN performed research; AH and HN analyzed data; HN and MSE wrote the paper. The authors declare that they are responsible for the article’s scientific content including study design, data collection, analysis and interpretation, writing, some of the main line, or all of the preparation and scientific review of the contents and approval of the final version of the article.

Corresponding authors

Correspondence to Hayrunnisa Nadaroglu or Ahmet Hacimuftuoglu.

Ethics declarations

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflicts of interests

The authors declare that there are no conflicts of interest regarding the publication of this study.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ertugrul, M.S., Nadaroglu, H., Nalci, O.B. et al. Preparation of CoS nanoparticles-cisplatin bio-conjugates and investigation of their effects on SH-SY5Y neuroblastoma cell line. Cytotechnology 72, 885–896 (2020). https://doi.org/10.1007/s10616-020-00432-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10616-020-00432-5

Keywords

Search

Navigation