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Bioactive molecule Icariin inhibited proliferation while enhanced apoptosis and autophagy of rat airway smooth muscle cells in vitro

  • Lihong Yang
  • Bo Xiao
  • Lixia Hou
  • Guiming Zhou
  • Biwen Mo
  • Dong YaoEmail author
Original Article
  • 67 Downloads

Abstract

Icariin is the main active compound extracted from epimedium Flavonoids (EFs) and involved in regulation of cell behaviors (proliferation, apoptosis, and autophagy etc.) for many cell types, but the effect of Icariin on airway smooth muscle cells (ASMCs) is still unknown. The aim of the present study is to examine the role of Icariin on rat ASMCs proliferation, apoptosis and autophagy. CKK8 assay showed that Icariin inhibited rat ASMCs growth in dose-time-dependent manner, and the flow cytometry assay showed that the Icariin interfered with ASMCs cell cycle, when treated with Icariin, S phase shortened while G2 phase extended, cyclin E1 and cyclinA1 gene and protein expression decreased. Next apoptosis was detected, Flow cytometry and TdTmediated dUTP Nick-End Labeling (TUNEL) assay showed that Icariin promoted ASMCs apoptosis, and enhanced apoptosis protein cleaved-caspase-3 expression. Finally, it was found Icariin induced rat ASMCs autophagy, with enhancement expression of autophagy marker LC3 II. In conclusion, Icariin inhibited ASMCs proliferation while promoted apoptosis and autophagy, revealing its potential role in treatment of airway remodeling in asthma.

Keywords

Icariin Airway smooth muscle cells Proliferation Apoptosis Autophagy 

Notes

Acknowledgements

We thank the staffs of Science Laboratory Center of Guilin Medical University for technical supports.

Funding

This study was supported by National Natural Science Foundation of China (Grant Nos. 81560005, 81760007) and Natural Science Foundation of Guangxi Province (2016GXNSFBA380003)

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Respiratory & Critical Care MedicineThe Affiliated Hospital of Guilin Medical UniversityGuilinChina
  2. 2.Key Laboratory of Respiratory Diseases of Colleges and Universities Affiliated Education Department of Guangxi Zhuang Autonomous RegionGuilinChina
  3. 3.Laboratory of Respiratory Disease, The Affiliated Hospital of Guilin Medical UniversityGuilinChina

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