Abstract
Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated β-galactosidase, p21, p16, phosphorylated p38, and γH2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression.
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Acknowledgements
The authors like to thank Dr. K. Fukami (Kyushu Univ.) and T. H. Bang (Kyushu Univ.) for their assistance.
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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
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Chong, Z., Matsuo, H., Kuroda, M. et al. Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes. Cytotechnology 70, 1001–1008 (2018). https://doi.org/10.1007/s10616-018-0229-1
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DOI: https://doi.org/10.1007/s10616-018-0229-1