Enhancement of osteogenic differentiation of adipose-derived stem cells by PRP modified nanofibrous scaffold
- 210 Downloads
Recent developments in bone tissue engineering have paved the way for more efficient and cost-effective strategies. Additionally, utilization of autologous sources has been considered very desirable and is increasingly growing. Recently, activated platelet rich plasma (PRP) has been widely used in the field of bone tissue engineering, since it harbours a huge number of growth factors that can enhance osteogenesis and bone regeneration. In the present study, the osteogenic effects of PRP coated nanofibrous PES/PVA scaffolds on adipose-derived mesenchymal stem cells have been investigated. Common osteogenic markers were assayed by real time PCR. Alkaline phosphate activity, calcium deposition and Alizarin red staining assays were performed as well. The results revealed that the highest osteogenic differentiation occurred when cells were cultured on PRP coated PES/PVA scaffolds. Interestingly, direct application of PRP to culture media had no additive effects on osteogenesis of cells cultured on PRP coated PES/PVA scaffolds or those receiving typical osteogenic factors. The highest osteogenic effects were achieved by the simplest and most cost-effective method, i.e. merely by using PRP coated scaffolds. PRP coated PES/PVA scaffolds can maximally induce osteogenesis with no need for extrinsic factors. The major contribution of this paper to the current researches on bone regeneration is to suggest an easy, cost-effective approach to enhance osteogenesis via PRP coated scaffolds, with no additional external growth factors.
KeywordsBone tissue engineering Adipose-derived mesenchymal stem cell PRP Nanofibrous scaffold
This work was supported financially by Stem Cell Technology Research Center.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- Amiri B, Ghollasi M, Shahrousvand M, Kamali M, Salimi A (2016) Osteoblast differentiation of mesenchymal stem cells on modi fi ed PES-PEG electrospun fi brous composites loaded with Zn 2 SiO 4 bioceramic nanoparticles. Differentiation 1–11. https://doi.org/10.1016/j.diff.2016.08.001
- Ardeshirylajimi A, Dinarvand P, Seyedjafari E, Langroudi L, Jamshidi Adegani F, Soleimani M (2013) Enhanced reconstruction of rat calvarial defects achieved by plasma-treated electrospun scaffolds and induced pluripotent stem cells. Cell Tissue Res 354:849–860. https://doi.org/10.1007/s00441-013-1693-8 CrossRefPubMedGoogle Scholar
- Ardeshirylajimi A, Farhadian S, Adegani FJ, Mirzaei S, Zomorrod MS, Langroudi L, Doostmohammadi A, Seyedjafari E, Soleimani M (2015) Enhanced osteoconductivity of polyethersulphone nanofibres loaded with bioactive glass nanoparticles in in vitro and in vivo models. Cell Prolif 48:455–464. https://doi.org/10.1111/cpr.12198 CrossRefPubMedGoogle Scholar
- Babaeijandaghi F, Shabani I, Seyedjafari E, Naraghi ZS, Vasei M, Haddadi-Asl V, Hesari KK, Soleimani M (2010) Accelerated epidermal regeneration and improved dermal reconstruction achieved by polyethersulfone nanofibers. Tissue Eng Part A 16:3527–3536. https://doi.org/10.1089/ten.tea.2009.0829 CrossRefPubMedGoogle Scholar
- Díaz-gómez L, Alvarez-lorenzo C, Concheiro A, Silva M, Sheikh FA, Cantu T, Desai R, Garcia VL (2015) NIH public access 180–188. https://doi.org/10.1016/j.msec.2014.03.065.Biodegradable
- Freitag J, Bates D, Boyd R, Shah K, Barnard A, Huguenin L, Tenen A (2016) Mesenchymal stem cell therapy in the treatment of osteoarthritis: reparative pathways, safety and efficacy – a review. BMC Musculoskelet. Disord. https://doi.org/10.1186/s12891-016-1085-9 CrossRefPubMedPubMedCentralGoogle Scholar
- Haddadi-asl V (2010) Prepared by electrospinning ch archive 19, pp 457–468Google Scholar
- Hanaee H, Masoud A, Hamid S (2012) Effective combination of hydrostatic pressure and aligned nanofibrous scaffolds on human bladder smooth muscle cells: implication for bladder tissue engineering 2281–2290. https://doi.org/10.1007/s10856-012-4688-1
- Kabiri M, Oraee-yazdani S, Dodel M, Hanaee-ahvaz H, Soudi S, Seyedjafari E, Salehi M, Soleimani M, Cell S, Cell S, Neurosurgery F, Hospital ST, Beheshti S, Cell S (2015) Original article : Cytocompatibility of a conductive nanofibrous carbon nanotube/poly (l-lactic acid) composite. EXCLI J 14:851–860PubMedPubMedCentralGoogle Scholar
- Manuscript A (2013) NIH public access 30, pp 546–554. https://doi.org/10.1016/j.tibtech.2012.07.005.Recent
- Pournaqi F, Farahmand M, Ardeshirylajimi A (2016) Increasing biocompatibility of scaffold made of polyethersulfone (PES) through combining with polyaniline (PANI). J Paramed Sci 7:1–6Google Scholar
- Schofer MD, Roessler PP, Schaefer J, Theisen C, Schlimme S, Heverhagen JT, Voelker M, Dersch R, Agarwal S, Fuchs S, Paletta RJ (2011) Electrospun PLLA nanofiber scaffolds and their use in combination with BMP-2 for reconstruction of bone defects. PLoS ONE. https://doi.org/10.1371/journal.pone.0025462 CrossRefPubMedPubMedCentralGoogle Scholar
- Sun H, Zhang Y, Dou L, Song X, Gu X, Fu C (2016) Nanofiber design for human stem cell culture. Rev Adv Mater Sci 44:160–167Google Scholar
- Unger RE, Peters K, Huang Q, Funk A, Paul D, Kirkpatrick CJ (2005) Vascularization and gene regulation of human endothelial cells growing on porous polyethersulfone (PES) hollow fiber membranes. Biomaterials 26:3461–3469. https://doi.org/10.1016/j.biomaterials.2004.09.047 CrossRefPubMedGoogle Scholar