, Volume 70, Issue 2, pp 843–854 | Cite as

Characterization of female germline stem cells from adult mouse ovaries and the role of rapamycin on them

  • Hong Yang
  • Xi Yao
  • Furong Tang
  • Yudong Wei
  • Jinlian Hua
  • Sha Peng
Original Article


Germline stem cells (GSCs) play an indispensable role in establishing the fertility of an organism. The isolation and culture of adult female GSCs (FGSCs) have provided a robust foundation to study the development of female germ cells in rodents. However, many problems still need to be identified, such as the origin and location of FGSCs and the specific markers for screening. In this study, we acquired FGSCs that stably expressed Oct4 from Oct4 promoter-GFP transgenic mouse ovarian surface epithelium and cortical layer, and identified the cells possessing the representative features including the expression of GSCs marker genes and the potentiality of differentiation into all three germ layers in vitro. Moreover, rapamycin was confirmed to promote proliferation of mouse FGSCs and inhibit the differentiation capability in vivo. In addition to the reported disinfection function, rapamycin inhibited the activation of primordial follicles, as the inhibitor of mechanistic target of rapamycin pathway. These results will contribute to the study on folliculogenesis or oogenesis mechanism and have important implications on developing new technology and therapeutic approach in medicine for premature ovarian failure, infertility and even ovary remodelling in future.


Female germline stem cells Characterization Rapamycin Mouse 



This study was supported by Basal Research Fund of Northwest A&F University (2452015034) and Agricultural Science and Technology Innovation and Key Project of Shaanxi Province (2016NY-094).

Supplementary material

10616_2018_196_MOESM1_ESM.docx (65 kb)
Supplementary material 1 (DOCX 64 kb)


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Hong Yang
    • 1
  • Xi Yao
    • 1
  • Furong Tang
    • 1
  • Yudong Wei
    • 1
  • Jinlian Hua
    • 1
  • Sha Peng
    • 1
  1. 1.College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering and TechnologyNorthwest A&F UniversityYanglingChina

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