Co-cultured the MSCs and cardiomyocytes can promote the growth of cardiomyocytes
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Recently, the incidence of myocardial infarction has been increasing annually. Now cell therapy is a major new strategy in the treatment of this public health challenge. Most recently, evidences showed that MSCs can reduce the area of infarction and improve the heart function. In our study we found that MSCs could promote cardiomyocytes proliferation, inhibit the apoptosis of cardiomyocytes and promote cardiomyocytes autophagy function. These functions could be a therapeutic effect on myocardial infarction. At the same time, we first revealed that MSCs may achieve these functions by the activation of VEGF signaling pathways.
KeywordsCardiomyocytes MSCs Myocardial infarction
Marrow mesenchyme stem cells
Vascular endothelial growth factor
- SD rat
Dulbecco’s Modified Eagle’s medium
Fetal bovine serum
Phosphate buffer saline
Reactive oxygen species
Flow cytometry assay
Thanks the care and support of all colleagues.
HJ: conceived of the study, carried out the molecular studies. HW: carried out the molecular studies, helped to draft the manuscript. TL: participated in the design of the study and performed the statistical analysis. ZY: participated in its design and coordination and helped to draft the manuscript. RZ: conceived of the study. HH: conceived of the study.
This research was sponsored by the Science and Technology Project of Liaoning Province (2013225089).
Compliance with ethical standards
Conflict of interest
No conflicts of interest. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Animal study received China medical university animal care and use committee approval.
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