Co-cultured the MSCs and cardiomyocytes can promote the growth of cardiomyocytes
Recently, the incidence of myocardial infarction has been increasing annually. Now cell therapy is a major new strategy in the treatment of this public health challenge. Most recently, evidences showed that MSCs can reduce the area of infarction and improve the heart function. In our study we found that MSCs could promote cardiomyocytes proliferation, inhibit the apoptosis of cardiomyocytes and promote cardiomyocytes autophagy function. These functions could be a therapeutic effect on myocardial infarction. At the same time, we first revealed that MSCs may achieve these functions by the activation of VEGF signaling pathways.
KeywordsCardiomyocytes MSCs Myocardial infarction
Marrow mesenchyme stem cells
Vascular endothelial growth factor
- SD rat
Dulbecco’s Modified Eagle’s medium
Fetal bovine serum
Phosphate buffer saline
Reactive oxygen species
Flow cytometry assay
Thanks the care and support of all colleagues.
HJ: conceived of the study, carried out the molecular studies. HW: carried out the molecular studies, helped to draft the manuscript. TL: participated in the design of the study and performed the statistical analysis. ZY: participated in its design and coordination and helped to draft the manuscript. RZ: conceived of the study. HH: conceived of the study.
Compliance with ethical standards
Conflict of interest
No conflicts of interest. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Animal study received China medical university animal care and use committee approval.
- Kajdaniuk D, Marek B, Borgiel-Marek H, Kos-Kudla B (2011) Vascular endothelial growth factor (VEGF)—part 1: in physiology and pathophysiology. Endokrynol Pol 62:444–455Google Scholar
- Shi C, Zhao Y, Yang Y, Chen C, Hou X, Shao J et al (2017) Collagen-binding VEGF targeting the cardiac extracellular matrix promotes recovery in porcine chronic myocardial infarction. Biomat SciGoogle Scholar
- Tan MY, Zhang CD, Xia B, Guo J, Fan ZW, Wu TH et al (2016) The expression of HMGB1 in bone marrow MSCs is upregulated by hypoxia with regulatory effects on the apoptosis and adhesion. Biomed Res Int 2016:4598927Google Scholar
- Yuan H, Zhang J, Zhang R (2013) Experimental study on adipose-derived stem cells transfected by bone morphogenetic protein 14 co-culture with chondrocytes. Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi. Chin J Reparative Reconstr Surg 27:353–357Google Scholar
- Zhang L, Desai NR, Li J, Hu S, Wang Q, Li X et al (2015) National quality assessment of early clopidogrel therapy in Chinese patients with acute myocardial infarction (AMI) in 2006 and 2011: insights from the China patient-centered evaluative assessment of cardiac events (PEACE)-retrospective AMI study. J Am Heart Assoc 4:e001906CrossRefGoogle Scholar