Characterisation and immunosuppressive activity of human cartilage-derived mesenchymal stem cells

Sandrasaigaran, Pratheep; Algraittee, Satar Jabbar Rahi; Ahmad, Azfar Rizal; Vidyadaran, Sharmili; Ramasamy, Rajesh

doi:10.1007/s10616-017-0182-4

Published: 01 March 2018

Characterisation and immunosuppressive activity of human cartilage-derived mesenchymal stem cells

Pratheep Sandrasaigaran^1,2,
Satar Jabbar Rahi Algraittee^1,3,
Azfar Rizal Ahmad⁴,
Sharmili Vidyadaran¹ &
Rajesh Ramasamy ORCID: orcid.org/0000-0003-4227-0458¹

Cytotechnology volume 70, pages 1037–1050 (2018)Cite this article

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Abstract

Mesenchymal stem cells (MSCs) exert potent immuno-regulatory activities on various immune cells and also differentiate into various mesodermal lineages besides retaining a distinct self-renewal ability. Such exclusive characteristics had enabled MSCs to be recognised as an ideal source for cell-based treatment in regenerative medicine and immunotherapy. Thus, considering MSCs for treating degenerative disease of organs with limited regenerative potential such as cartilage would serve as an ideal therapy. This study explored the feasibility of generating human cartilage-derived MSCs (hC-MSCs) from sports injured patients and characterised based on multipotent differentiation and immunosuppressive activities. Cartilage tissues harvested from a non-weight bearing region during an arthroscopy procedure were used to generate MSCs. Despite the classic morphology of fibroblast-like cells and a defined immunophenotyping, MSCs expressed early embryonic transcriptional markers (SOX2, REX1, OCT4 and NANOG) and differentiated into chondrocytes, adipocytes and osteocytes when induced accordingly. Upon co-culture with PHA-L activated T-cells, hC-MSCs suppressed the proliferation of the T-cells in a dose-dependent manner. Although, hC-MSCs did not alter the activation profile of T cells significantly, yet prevented the entering of activated T cells into S phase of the cell cycle by cell cycle arrest. The present study has strengthened the evidence of tissue-resident mesenchymal stem cells in human cartilage tissue. The endogenous MSCs could be an excellent tool in treating dysregulated immune response that associated with cartilage since hC-MSCs exerted both immunosuppressive and regenerative capabilities.

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Abbreviations

MSCs:: Mesenchymal stem cells

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Acknowledgements

This project supported by Research University Grant Scheme (RUGS) 04-02-12-1757RU and 04/02/10/0920RU by Universiti Putra internal grant scheme. The postgraduate student, Pratheep Sandrasaigaran partially supported by special research graduate scheme by Universiti Putra Malaysia.

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Authors and Affiliations

Stem Cell and Immunity Research Group, Immunology Laboratory, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia
Pratheep Sandrasaigaran, Satar Jabbar Rahi Algraittee, Sharmili Vidyadaran & Rajesh Ramasamy
School of Science and Engineering, Manipal International University, No 1, MIU Boulevard, 71800, Putra Nilai, Negeri Sembilan, Malaysia
Pratheep Sandrasaigaran
Department of Medical Microbiology, College of Medicine, Kerbala University, Kerbala, 46001, Iraq
Satar Jabbar Rahi Algraittee
Columbia Asia Hospital Cheras, Lot 33107, Jalan Suakasih, 43200, Cheras, Selangor, Malaysia
Azfar Rizal Ahmad

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Pratheep Sandrasaigaran
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Satar Jabbar Rahi Algraittee
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Azfar Rizal Ahmad
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Sharmili Vidyadaran
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Rajesh Ramasamy
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Correspondence to Rajesh Ramasamy.

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Sandrasaigaran, P., Algraittee, S.J.R., Ahmad, A.R. et al. Characterisation and immunosuppressive activity of human cartilage-derived mesenchymal stem cells. Cytotechnology 70, 1037–1050 (2018). https://doi.org/10.1007/s10616-017-0182-4

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Received: 21 September 2017
Accepted: 07 December 2017
Published: 01 March 2018
Issue Date: June 2018
DOI: https://doi.org/10.1007/s10616-017-0182-4

Keywords

Mesenchymal
Cartilage
Sports injuries
Cell differentiation
Cell cycle