Comparable osteogenic capacity of mesenchymal stem or stromal cells derived from human amnion membrane and bone marrow
So far, substantial attentions have been attracted to the application of mesenchymal stem or stromal cells (MSCs) in different therapeutic approaches. Although human bone marrow is commonly considered as a major source for MSCs, having an invasive collection method, ethical consideration and donor availability create a challenge for scientists, leading them to explore better alternative sources for MSCs. The study presented here aimed to characterize and compare osteogenic capacity of MSCs obtained from the amnion membrane (AM) with those originated from BM. Cells isolated from AMs and BMs were cultured in DMEM-low glucose supplemented with FBS, penicillin and streptomycin. After 24 h of incubation, cells adhered to the plastic surface of the flasks were allowed to proliferate for more days. A sub-confluent culture of cells was trypsinized and re-cultured. The MSCs were characterized by the expression of specific markers with flow cytometry. The osteogenic differentiation of MSCs was also validated by alkaline phosphatase and alizarian red S staining. Our results showed comparable expression of MSCs specific markers for both MSC sources (AM and BM). We also showed the optimum osteogenic differentiation of MSCs from both sources whereas hAM-MSCs revealed higher proliferation rate. We found no essential immunophenotypic differences between MSCs originated from bone marrow and amnion membrane while their differentiations into osteoblastic linage were also comparable. This was in addition to the higher proliferation rate observed for hAM-MSCs which suggests hAM as an easily accessible and reliable source of MSCs applicable for bone engineering, regenerative medicine or other therapeutic approaches.
KeywordsAmnion membrane Bone marrow Mesenchymal stem or stromal cells Osteogenic
This work was part of Dr. Hosseini’s approved project (No. 1390-01-33-1516) supported by Iranian blood transfusion organization and High Institute for Research and Education in Transfusion Medicine in Iran. The authors declare no conflict of interests.
- Barry FP (2003) Mesenchymal stem cell therapy in joint disease. Novartis Found Symp 249:86–96 (discussion 96–102, 170–104, 239–141) Google Scholar
- Chen WM, Chen ZX et al (2008) Osteoblasts from patients with myelodysplastic syndrome express multiple cytokines and support hematopoietic progenitor cell survival in vitro. Zhongguo Shi Yan Xue Ye Xue Za Zhi 16:78–83Google Scholar
- Du X, Yuan Q et al (2016) Endometrial mesenchymal stem cells isolated from menstrual blood by adherence. Stem Cells Int 2016:3573846Google Scholar
- Huang XB, Liu T et al (2006) Osteoblasts differentiated from human marrow bone mesenchymal stem cells support hematopoietic stem/progenitor cells from umbilical cord blood. Zhongguo Shi Yan Xue Ye Xue Za Zhi 14:552–556Google Scholar
- Kilic E, Ceyhan T et al (2007) Evaluation of differentiation potential of human bone marrow-derived mesenchymal stromal cells to cartilage and bone cells. Acta Orthop Traumatol Turc 41:295–301Google Scholar
- Kuo Ching Chao KFC (2008) Islet-like clusters derived from mesenchymal stem cells in Wharton’s jelly of the human umbilical cord for transplantation to control type 1 diabetes. Public Library of ScienceGoogle Scholar
- Manochantr S, Tantrawatpan C et al (2010) Isolation, characterization and neural differentiation potential of amnion derived mesenchymal stem cells. J Med Assoc Thai 93:S183–S191Google Scholar
- Miura Y (2013) Regulation of hematopoiesis by mesenchymal stem cells. Rinsho Ketsueki 54:431–435Google Scholar
- Mobasheri A, Csaki C et al (2009) Mesenchymal stem cells in connective tissue engineering and regenerative medicine: applications in cartilage repair and osteoarthritis therapy. Histol Histopathol 24:347–366Google Scholar
- Shu J, Guo L et al (2011) Experimental study on the induced differentiation of human amnion mesenchymal cells into osteoblasts. Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chin J Plast Surg 27:362–367Google Scholar
- Verfaillie CM (1998) Adhesion receptors as regulators of the hematopoietic process. Blood 92:2609–2612Google Scholar
- Wang Y, Jiang F et al (2016a) Human amnion-derived mesenchymal stem cells promote osteogenic differentiation in human bone marrow mesenchymal stem cells by influencing the ERK1/2 signaling pathway. Stem Cells Int 2016:4851081Google Scholar