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Cytotechnology

, Volume 70, Issue 2, pp 701–711 | Cite as

Promoting effects of adipose-derived stem cells on breast cancer cells are reversed by radiation therapy

  • Annemarie Baaße
  • Dajana Juerß
  • Elaine Reape
  • Katrin Manda
  • Guido Hildebrandt
Article

Abstract

Partial breast irradiation of early breast cancer patients after lumpectomy and the use of endogenous adipose tissue (AT) for breast reconstruction are promising applications to reduce the side effects of breast cancer therapy. This study tries to investigate the possible risks associated with these therapeutic approaches. It also examines the influence of adipose derived stem cells (ADSCs) as part of the breast cancer microenvironment, and endogenous AT on breast cancer cells following radiation therapy. ADSCs, isolated from human reduction mammoplasties of healthy female donors, exhibited multilineage capacity and specific surface markers. The promoting effects of ADSCs on the growth and survival fraction of breast cancer cells were reversed by treatment with high (8 Gy) or medium (2 Gy) radiation doses. In addition, a suppressing influence on breast cancer growth could be detected by co-culturing with irradiated ADSCs (8 Gy). Furthermore the clonogenic survival of unirradiated tumor cells was reduced by medium of irradiated ADSCs. In conclusion, radiation therapy changed the interactions of ADSCs and breast cancer cells. On the basis of our work, the importance of further studies to exclude potential risks of ADSCs in regenerative applications and radiotherapy has been emphasized.

Keywords

Adipose-derived stem cells Radiation Radiation-induced bystander effect Breast cancer 

Abbreviations

7-AAD

7-Aminoactinomycin

ADSCs

Adipose-derived stem cells

AT

Adipose Tissue

BrdU

Bromodeoxyuridine

CM

Conditioned medium

DMEM-F12

Dulbecco’s modified Eagle medium and Kaighn's modification of Ham’s F12

DMSO

Dimethyl sulfoxide

FBS

Fetal bovine serum Superior

MT

Medium transfer

NSABP

National Surgical Adjuvant Breast and Bowel Project

P/S

Penicillin/streptomycin

PBS

Phosphate-buffered saline

PE

Plating efficiency

RIBEs

Radiation-induced bystander effects

SD

Standard deviation

SEM

Standard error of mean

SF

Survival fraction

Notes

Acknowledgements

Human breast tissue from cosmetic reduction mammoplasties were very kindly provided by Prof. Bernd Gerber at the Department of Obstetrics and Gynecology, University Medical Center Rostock and Dr. Jürgen Weber at the Clinic of Aesthetics of the Academy for Transdermal Delivery Research e.V., Rostock, as well as Prof. Dr. Björn Dirk Krapohl, specialist in plastic and hand surgery of the St. Marien Hospital, Berlin and Prof. Dr. Peter Mailänder, head of plastic surgery at the UKSH, Lübeck.

Compliance with ethical standards

Conflict of interests

The authors have no conflict of interest to declare.

Supplementary material

10616_2017_172_MOESM1_ESM.docx (785 kb)
Supplementary material 1 (DOCX 784 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Annemarie Baaße
    • 1
  • Dajana Juerß
    • 1
  • Elaine Reape
    • 1
  • Katrin Manda
    • 1
  • Guido Hildebrandt
    • 1
  1. 1.Department of Radiotherapy and Radiation OncologyUniversity Medical Centre RostockRostockGermany

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