, Volume 70, Issue 1, pp 465–477 | Cite as

PDGFR inhibition mediated intracellular signalling in C6 glioma growth and migration: role of ERK and ROCK pathway

Original Article


Aberrant PDGFR (Platelet derived growth factor receptor) signalling in brain tumors and gliomas is one of the primary cause of tumor progression. PDGFR stimulation by its ligand and the role of its downstream mediators such as extracellular regulated kinases (ERK1/2), PI3K and ROCK pathways have not been thoroughly investigated. The present study sought to investigate the role of PDGF receptor signalling inhibition on suppression of rat C6 glioma growth and migration. Treatment of C6 cells with PDGFR inhibitor, AG1295 caused a significant reduction in migration and proliferation by regulating the ERK and ROCK signalling. Subsequently, PDGFR blocking was demonstrated to regulate cytoskeleton reorganization by modulating the Actin-pMLC reorganization and pERK–FAK–Paxillin complex formation which may further regulate the C6 glioma migration. Further, other malignant behaviour of C6 glioma such as anchorage independent growth, adhesion, invasion and sphere forming abilities were found to be impaired by PDGFR blocking. PDGFR inhibition further regulates the C6 glioma tumor behaviour by inducing gene expression of GFAP, BDNF, and MECP2 and down regulating FAK expression. In conclusion, our data elucidate novel mechanisms involve in PDGFR inhibition mediated inhibition of C6 glioma growth and migration which can be a future potential target for the treatment of glioma.


C6 glioma Migration PDGFR ERK ROCK 



This work was funded by a grant from University Grant Commission (F. No.41-148/2012 (SR) dated 13th July 2012), New Delhi, India. Currently, Ms. Juhi Singh is being supported by fellowship grant awarded by CSIR-SRF, New Delhi (CSIR-SRF Project No: 09/114(0206)/2016-EMR-I dated 31-3-17). We also thankful to DBT-MSUB-ILSPARE programme for the confocal microscopy and qPCR facility at Dr. Vikram Sarabhai Science Block, Faculty of Science, The M.S University of Baroda, Vadodara.

Compliance with ethical standards

Conflict of interest

The Authors report no conflict of interest.


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Juhi Singh
    • 1
  • Kedarlal Sharma
    • 1
  • Prakash P. Pillai
    • 1
  1. 1.Department of Zoology, Faculty of ScienceMaharaja Sayajirao University of BarodaVadodaraIndia

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