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Cytotechnology

, Volume 70, Issue 1, pp 431–438 | Cite as

Therapeutic effect of 28-homobrassinolide on leukotriene synthesis in leukemia cells

  • Chuan He
  • Zhigang Liu
  • Jingcao Huang
  • Ting Liu
Original Article
  • 71 Downloads

Abstract

The present study was aimed to investigate the effect of 28-homobrassinolide on leukotriene synthesis in the 2H3 cells. Cell viability was determined by using sulforhodamine B (SRB) assay. Leukotriene C4 (LTC4) and Leukotriene B4 (LTB4) were determined in the cell and cell lysates. Intracellular Ca2+ was determined in the intact cells. Phospholipases A2 (PLA2) expression was determined in the cells using immunofluorescence. LTC4 and LTB4 were tremendously increased in IgE-loaded 2H3 cells. However, these levels were significantly reduced following treatment with 28-homobrassinolide. The intracellular Ca2+ level was not altered by treatment. Expression of PLA2 was significantly reduced following treatment with 28-homobrassinolide. Taking all these data together, it is suggested that the 28-homobrassinolide may be a potential therapeutic agent to inhibit leukotriene synthesis in 2H3 cells.

Keywords

28-homobrassinolide Leukotrienes Phospholipases A2 2H3 cells 

Notes

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Chuan He
    • 1
  • Zhigang Liu
    • 1
  • Jingcao Huang
    • 1
  • Ting Liu
    • 1
  1. 1.Department of Hematology, Hematology Research LaboratoryWest China Hospital, Sichuan UniversityChengduChina

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