Transplantation of mesenchymal stem cells modulated Cx43 and Cx45 expression in rats with myocardial infarction
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At present, little is known about the influence of mesenchymal stem cell (MSC) transplantation on connexin43 (Cx43) and connexin45 (Cx45) remodeling in the ischemic heart. In this study, we investigated the effect of MSC transplantation on Cx43 and Cx45 remodeling in the ischemic heart. Wistar rats were subjected to left anterior descending artery ligation to induce myocardial infarction (MI) and then randomly allocated to receive an intramyocardial injection of PBS (MI group) or 5-azacytidine-induced MSCs (MSCs group). Histological examination and western blotting were performed 4 weeks after cell transplantation. We found that the MSCs exhibited plasticity by differentiating into cardiomyocyte-like cells. Gap junction remodeling after MI was characterized by a decrease in Cx43 expression and an increase in Cx45 expression. MSC transplantation modulated the MI-induced abnormalities by up-regulating Cx43 and down-regulating Cx45 expression. MSCs exhibited plasticity by differentiating into cardiomyocyte-like cells and modulated abnormal Cx43 and Cx45 remodeling following MI.
KeywordsMesenchymal stem cells Connexins Myocardial infarction
We would like to thank Drs. Ying-lin Wu and Jing-bo Jiang for their manuscript edits and revisions.
This study was supported by the National Natural Science Foundation of China (No. 30560051).
Compliance with ethical standards
Conflict of interest
- Li J-Y, He Y, Wen L, Ke HH, Wei Z, Deng Y, Wu ZF (2009a) Effects of allogenic bone marrow mesenchymal stem cell transplantation on electrophysiological abnormality and left ventricular remodeling in rats with myocardial infarction. J Clin Rehabil Tissue Eng Res 13:5211–5216Google Scholar
- Li J-Y, Zong GQ, Wei Z, Xu WY, Ke HH, Ning Z, Wu ZF (2009b) Establishment of a rat model of myocardial infarction and the post-infarction changes in electrophysiology and left ventricular function. Acta Lab Anim Sci Sin 17:419–423Google Scholar
- Roell W, Lewalter T, Sasse P, Tallini YN, Choi BR, Breitbach M, Doran R, Becher UM, Hwang SM, Bostani T, von Maltzahn J, Hofmann A, Reining S, Eiberger B, Gabris B, Pfeifer A, Welz A, Willecke K, Salama G, Schrickel JW, Kotlikoff MI, Fleischmann BK (2007) Engraftment of connexin 43-expressing cells prevents post-infarct arrhythmia. Nature 450:819–824CrossRefGoogle Scholar
- Smith JH, Green CR, Peters NS, Rothery S, Severs NJ (1991) Altered patterns of gap junction distribution in ischemic heart disease. An immunohistochemical study of human myocardium using laser scanning confocal microscopy. Am J Pathol 139:801–821Google Scholar
- Zhao YM, Li JY, He Y, Ke HH, Wang DX (2009) mRNA expression of connectin 43 and connectin 45 following transplantation of allogenic bone marrow mesenchymal stem cells in rats with acute myocardial infarction. J Clin Rehab Tissue Eng Res 13:8895–8900Google Scholar
- Zhong G, Moreno AP (2002) The formation of mono-heteromeric Cx43-Cx45/43 gap junctions uncovers gating and selectivity properties of their channels. Biophys J 82:633bGoogle Scholar