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Cytotechnology

, Volume 70, Issue 1, pp 225–234 | Cite as

Transplantation of mesenchymal stem cells modulated Cx43 and Cx45 expression in rats with myocardial infarction

  • Jin-yi Li
  • Hong-hong Ke
  • Yan He
  • Li-na Wen
  • Wei-yan Xu
  • Zhi-fu Wu
  • Yan-mei Zhao
  • Guo-qiang Zhong
Original Article
  • 126 Downloads

Abstract

At present, little is known about the influence of mesenchymal stem cell (MSC) transplantation on connexin43 (Cx43) and connexin45 (Cx45) remodeling in the ischemic heart. In this study, we investigated the effect of MSC transplantation on Cx43 and Cx45 remodeling in the ischemic heart. Wistar rats were subjected to left anterior descending artery ligation to induce myocardial infarction (MI) and then randomly allocated to receive an intramyocardial injection of PBS (MI group) or 5-azacytidine-induced MSCs (MSCs group). Histological examination and western blotting were performed 4 weeks after cell transplantation. We found that the MSCs exhibited plasticity by differentiating into cardiomyocyte-like cells. Gap junction remodeling after MI was characterized by a decrease in Cx43 expression and an increase in Cx45 expression. MSC transplantation modulated the MI-induced abnormalities by up-regulating Cx43 and down-regulating Cx45 expression. MSCs exhibited plasticity by differentiating into cardiomyocyte-like cells and modulated abnormal Cx43 and Cx45 remodeling following MI.

Keywords

Mesenchymal stem cells Connexins Myocardial infarction 

Notes

Acknowledgements

We would like to thank Drs. Ying-lin Wu and Jing-bo Jiang for their manuscript edits and revisions.

Funding

This study was supported by the National Natural Science Foundation of China (No. 30560051).

Compliance with ethical standards

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Jin-yi Li
    • 1
  • Hong-hong Ke
    • 1
  • Yan He
    • 1
  • Li-na Wen
    • 1
  • Wei-yan Xu
    • 1
  • Zhi-fu Wu
    • 1
  • Yan-mei Zhao
    • 1
  • Guo-qiang Zhong
    • 1
  1. 1.Department of CardiologyThe First Affiliated Hospital of Guangxi Medical UniversityNanningChina

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