Cytotechnology

, Volume 70, Issue 1, pp 163–167 | Cite as

Investigations on in vitro anti-carcinogenic potential of l-carnosine in liver cancer cells

  • Minghui Ding
  • Guihua Jiao
  • Haizhou Shi
  • Yanrong Chen
Original Article

Abstract

This study was carried out to investigate the anti-carcinogenic effect of l-carnosine in human carcinoma cells (SNU-423). The SNU-423 cancer cells were cultured at a density of 2 × 104 cells/well in Dulbecco modified Eagle medium. After 24 h of adherence, the cells were treated with l-carnosine (0.2 and 1 mg/mL) for 48 h. Then, cell viability was assessed by sulforhodamine assay, while mitochondrial dysfunction was measured by fluorescence microscopy using chromatin-specific dye Hoechst 33258. Intracellular levels of ROS were assayed by fluorescence spectroscopy with 2′,7′-dichlorofluorescein diacetate (DCFDA). l-Carnosine significantly inhibited the growth of the SNU-423 cells (p < 0.05). The inhibitory effect of l-carnosine was confirmed by results from mitochondrial fragmentation assay. The relative fluorescent unit was increased in a dose-dependent manner by l-carnosine, with values of 79.43, 186.87 and 400.89 for 0.6, 0.8 and 1 mg/mL of l-carnosine, respectively (p < 0.05). These results demonstrate that l-carnosine exerts anti-carcinogenic effects in human liver cancer cells.

Keywords

l-Carnosine Liver cancer ROS Inhibition Natural substance 

Notes

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Human participants and animals participants

Research involving Human Participants and/or Animals: This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Minghui Ding
    • 1
  • Guihua Jiao
    • 2
  • Haizhou Shi
    • 3
  • Yanrong Chen
    • 4
  1. 1.Department of Hepatobiliary SurgeryQingdao Municipal HospitalQingdaoChina
  2. 2.Department of Emergency CareFirst People’s Hospital of JinanJinanChina
  3. 3.Department of General SurgeryBinzhou Central HospitalBinzhouChina
  4. 4.Department of NeurologyBinzhou Central HospitalBinzhouChina

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