, Volume 70, Issue 1, pp 153–161 | Cite as

Glioma targeting peptide in combination with the P53 C terminus inhibits glioma cell proliferation in vitro

Original Article


Glioma is a prevalent malignant primary brain tumor in adults, the treatment for which remains a challenge due to its high infiltration and recurrence. Hence, treatments that lead to the suppression of glioma cell migration and invasion may be used in addition to surgery to increase the therapeutic outcome. In this study, we aimed to construct a multifunctional protein that would exert an effect on glioma cell proliferation and migration. The protein is named GL1-P53C-11R and it consists of the glioma-targeting peptide GL1 (G), the P53 C terminus (Pc) and the cell-penetrating peptide arginine (R). GL1-P53C-R was expressed with the fusion protein ZZ and immunofluorescence analysis showed effective delivery of the fused ZZ-GL1-P53C-R protein represented as ZZ-GPcR. The ZZ-GPcR exhibited an inhibitory effect on the proliferation, migration and invasion of U87ΔEGFR cells. Western blotting results indicated that it caused significant changes in the expression levels of cell cycle and apoptotic proteins. Flow cytometric analysis showed increase apoptosis. Our findings suggest that the P53C in the fusion protein ZZ-GPcR can enter into glioma cells to exert its inhibitory effect.


Glioma Targeting peptide P53 C terminus Cell proliferation Apoptosis 



This work was supported by the National Natural Science Foundation of China (Grant Nos. 81371676, 81071248) and the fundamental research fund of the key laboratory of Liaoning Provincial Education Department (LZ2015049).

Compliance with ethical standards

Conflict of interest

All authors declare no conflict of interests.


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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Dan Wang
    • 1
  • Meihua Guo
    • 1
  • Jiawen Yu
    • 1
  • Xinying Wang
    • 1
  • Qian Zhang
    • 1
  • Xu Yang
    • 1
  • Jiaqi Li
    • 1
  • Chunhui Zhao
    • 2
  • Bin Feng
    • 1
  1. 1.Department of BiotechnologyDalian Medical UniversityDalianChina
  2. 2.College of Life SciencesLiaoning Normal UniversityDalianChina

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