, Volume 70, Issue 2, pp 523–536 | Cite as

Spirulina platensis prevents high glucose-induced oxidative stress mitochondrial damage mediated apoptosis in cardiomyoblasts

  • Pratiksha Jadaun
  • Dhananjay Yadav
  • Prakash Singh Bisen
Original Article


The current study was undertaken to study the effect of Spirulina platensis (Spirulina) extract on enhanced oxidative stress during high glucose induced cell death in H9c2 cells. H9c2 cultured under high glucose (33 mM) conditions resulted in a noteworthy increase in oxidative stress (free radical species) accompanied by loss of mitochondrial membrane potential, release of cytochrome c, increase in caspase activity and pro-apoptotic protein (Bax). Spirulina extract (1 μg/mL), considerably inhibited increased ROS and RNS levels, reduction in cytochrome c release, raise in mitochondrial membrane potential, decreased the over expression of proapoptotic protein Bax and suppressed the Bax/Bcl2 ratio with induced apoptosis without affecting cell viability. Overall results suggest that Spirulina extract plays preventing role against enhanced oxidative stress during high glucose induced apoptosis in cardiomyoblasts as well as related dysfunction in H9c2 cells.

Graphical Abstract


H9c2 High glucose Oxidative stress Apoptosis Spirulina platensis 



Normal glucose


High glucose


Spirulina platensis


Reactive oxygen species


Reactive nitrogen species




Hydrogen peroxide


Nitric oxide



X + XO

Xanthine plus xanthine oxidase






Copper–Zinc-superoxide dismutase


NG-nitro-l-arginine methyl ester



We thank, Director,  National Centre of Cell Sciences, NCCS Complex, S.P. Pune University, Ganeshkhind, Pune, Maharashtra, India for providing basic facilities and to Dr. Sandhya Sitasawad, Senior Scientist, National Centre of Cell Sciences, NCCS Complex, S.P. Pune University, Ganeshkhind, Pune, Maharashtra, India for her critical suggestion, support and encouragement. This research work was supported by a research grant from Department of Science and Technology, Govt. of India, New Delhi under FTYS scheme   (SR/FT/LS-093/2007).

Compliance with ethical standards

Conflict of interest

We declare that there is no conflict of interest.

Supplementary material

10616_2017_121_MOESM1_ESM.pptx (684 kb)
Supplementary material 1 (PPTX 684 kb)
10616_2017_121_MOESM2_ESM.docx (13 kb)
Supplementary material 2 (DOCX 12 kb)


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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Pratiksha Jadaun
    • 1
  • Dhananjay Yadav
    • 2
  • Prakash Singh Bisen
    • 3
  1. 1.School of Studies in BiotechnologyGwaliorIndia
  2. 2.Department of Medical BiotechnologyYeungnam UniversityGyeongsanKorea
  3. 3.School of Studies in BiotechnologyJiwaji UniversityGwaliorIndia

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