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Cardiotrophin-1 stimulates the neural differentiation of human umbilical cord blood-derived mesenchymal stem cells and survival of differentiated cells through PI3K/Akt-dependent signaling pathways

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Abstract

Cardiotrophin-1 (CT1) plays an important role in the differentiation, development, and survival of neural stem cells. In this study, we analyzed its effects on the stimulation of human umbilical cord blood-derived mesenchymal stem cells in terms of their potential to differentiate into neuron-like cells, their survival characteristics, and the molecular mechanisms involved. The treatment of cells with neural induction medium (NIM) and CT1 generated more cells that were neuron-like and produced stronger expression of neural-lineage markers than cells treated with NIM and without CT1. Bcl-2 and Akt phosphorylation (p-Akt) expression levels increased significantly in cells treated with both NIM and CT1. This treatment also effectively blocked cell death following neural induction and decreased Bax, Bak and cleaved-caspase 3 expression compared with cells treated with NIM without CT1. In addition, the inhibition of phosphatidylinositol 3-kinase (PI3K) abrogated p-Akt and Bcl-2 expression. Thus, PI3K/Akt contribute to CT1-stimulated neural differentiation and to the survival of differentiated cells.

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Acknowledgements

This study was funded by Grants from the National Natural Science Foundation (No. 31260286) of China. We are grateful to doctors and nurses of the Obstetrical Department of Maternity and child health care hospital in Zunyi for the collection of the cord blood.

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Authors and Affiliations

  1. Department of Pediatric, First Affiliated Hospital, Zunyi Medical College, Zunyi, 563003, Guizhou Province, China

    Longying Peng, Xiaomei Shu, Changhui Lang & Xiaohua Yu

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  1. Longying Peng
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  2. Xiaomei Shu
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  3. Changhui Lang
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  4. Xiaohua Yu
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Correspondence to Xiaomei Shu.

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Peng, L., Shu, X., Lang, C. et al. Cardiotrophin-1 stimulates the neural differentiation of human umbilical cord blood-derived mesenchymal stem cells and survival of differentiated cells through PI3K/Akt-dependent signaling pathways. Cytotechnology 69, 933–941 (2017). https://doi.org/10.1007/s10616-017-0103-6

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  • DOI: https://doi.org/10.1007/s10616-017-0103-6

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