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Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro

Cytotechnology volume 69, pages 815–829 (2017)Cite this article

Abstract

Mollusc shells are composed of more than 95% calcium carbonate and less than 5% organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. In this study, we investigated the effects of matrix macromolecular components extracted from the shells of two edible molluscs of economic interest, i.e., the blue mussel Mytilus edulis and the Pacific oyster Crassostrea gigas. The potential biological activities of these organic molecules were analysed on human dermal fibroblasts in primary culture. Our results demonstrate that shell extracts of the two studied molluscs modulate the metabolic activities of the cells. In addition, the extracts caused a decrease of type I collagen and a concomitant increase of active MMP-1, both at the mRNA and the protein levels. Therefore, our results suggest that shell extracts from M. edulis and C. gigas contain molecules that promote the catabolic pathway of human dermal fibroblasts. This work emphasises the potential use of these shell matrices in the context of anti-fibrotic strategies, particularly against scleroderma. More generally, it stresses the usefulness to valorise bivalve shells that are coproducts of shellfish farming activity.

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Acknowledgements

The authors thank COPALIS (Boulogne-Sur-Mer, France) for providing shell powders, and Elsevier Language Editing Services for English revision. The project received the label of pole AQUIMER (M. Coquelle). This work was financially supported by the “Fonds Unique Interministériel” (FUI, French ministry of Economy and Industry, SEMINEROIL program) [09 2 90 6042]. MB were recipients of a PhD fellowship from the FUI and the Conseil Régional de Basse-Normandie [917RB103].

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Affiliations

  1. Normandie Université, CS 14032, Caen, 14032, France

    Thomas Latire, Florence Legendre, Mouloud Bouyoucef, Jean-Marc Lebel, Philippe Galéra & Antoine Serpentini

  2. UMR BOREA «Biologie des ORganismes et Ecosystèmes Aquatiques», MNHN, UPMC, UCBN, CNRS-7208, IRD-207, SFR 4206 ICORE, Université de Caen Normandie, IBFA, Campus 1, Science C, Esplanade de la Paix, CS 14032, 14032, Caen Cedex 5, France

    Thomas Latire, Jean-Marc Lebel & Antoine Serpentini

  3. Laboratoire Microenvironnement cellulaire et pathologies (MILPAT), EA 4652 and Biotargen EA7450, SFR 4206 ICORE, Faculté de Médecine, Université de Caen Normandie, CHU niveau 3, Avenue de la Côte de Nacre, CS 14032, 14032, Caen Cedex 5, France

    Thomas Latire, Florence Legendre, Mouloud Bouyoucef & Philippe Galéra

  4. UMR 6282 CNRS “Biogéosciences”, Université de Bourgogne Franche-Comté (UBFC), 6 Boulevard. Gabriel, 21000, Dijon, France

    Frédéric Marin

  5. Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules (ERRMECe), EA 1391, Institut des Matériaux, Université de Cergy-Pontoise, 2 avenue Adolphe-Chauvin, F-95000, Cergy-Pontoise Cedex, France

    Franck Carreiras

  6. Department of Plastic and Reconstructive Surgery, St-Martin Clinic, 14000, Caen, France

    Muriel Rigot-Jolivet

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  1. Thomas Latire
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  2. Florence Legendre
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  3. Mouloud Bouyoucef
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  4. Frédéric Marin
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  9. Antoine Serpentini
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Correspondence to Antoine Serpentini.

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Latire, T., Legendre, F., Bouyoucef, M. et al. Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro. Cytotechnology 69, 815–829 (2017). https://doi.org/10.1007/s10616-017-0096-1

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  • DOI: https://doi.org/10.1007/s10616-017-0096-1

Keywords

  • Biological activity
  • Extracellular matrix
  • Fibroblast
  • Mollusc
  • Shell matrix