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Cytotechnology

, Volume 69, Issue 5, pp 815–829 | Cite as

Shell extracts of the edible mussel and oyster induce an enhancement of the catabolic pathway of human skin fibroblasts, in vitro

  • Thomas Latire
  • Florence Legendre
  • Mouloud Bouyoucef
  • Frédéric Marin
  • Franck Carreiras
  • Muriel Rigot-Jolivet
  • Jean-Marc Lebel
  • Philippe Galéra
  • Antoine SerpentiniEmail author
Original Article

Abstract

Mollusc shells are composed of more than 95% calcium carbonate and less than 5% organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. In this study, we investigated the effects of matrix macromolecular components extracted from the shells of two edible molluscs of economic interest, i.e., the blue mussel Mytilus edulis and the Pacific oyster Crassostrea gigas. The potential biological activities of these organic molecules were analysed on human dermal fibroblasts in primary culture. Our results demonstrate that shell extracts of the two studied molluscs modulate the metabolic activities of the cells. In addition, the extracts caused a decrease of type I collagen and a concomitant increase of active MMP-1, both at the mRNA and the protein levels. Therefore, our results suggest that shell extracts from M. edulis and C. gigas contain molecules that promote the catabolic pathway of human dermal fibroblasts. This work emphasises the potential use of these shell matrices in the context of anti-fibrotic strategies, particularly against scleroderma. More generally, it stresses the usefulness to valorise bivalve shells that are coproducts of shellfish farming activity.

Keywords

Biological activity Extracellular matrix Fibroblast Mollusc Shell matrix 

Notes

Acknowledgements

The authors thank COPALIS (Boulogne-Sur-Mer, France) for providing shell powders, and Elsevier Language Editing Services for English revision. The project received the label of pole AQUIMER (M. Coquelle). This work was financially supported by the “Fonds Unique Interministériel” (FUI, French ministry of Economy and Industry, SEMINEROIL program) [09 2 90 6042]. MB were recipients of a PhD fellowship from the FUI and the Conseil Régional de Basse-Normandie [917RB103].

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Thomas Latire
    • 1
    • 2
    • 3
  • Florence Legendre
    • 1
    • 3
  • Mouloud Bouyoucef
    • 1
    • 3
  • Frédéric Marin
    • 4
  • Franck Carreiras
    • 5
  • Muriel Rigot-Jolivet
    • 6
  • Jean-Marc Lebel
    • 1
    • 2
  • Philippe Galéra
    • 1
    • 3
  • Antoine Serpentini
    • 1
    • 2
    Email author
  1. 1.Normandie UniversitéCaenFrance
  2. 2.UMR BOREA «Biologie des ORganismes et Ecosystèmes Aquatiques», MNHN, UPMC, UCBN, CNRS-7208, IRD-207, SFR 4206 ICOREUniversité de Caen NormandieCaen Cedex 5France
  3. 3.Laboratoire Microenvironnement cellulaire et pathologies (MILPAT), EA 4652 and Biotargen EA7450, SFR 4206 ICORE, Faculté de MédecineUniversité de Caen NormandieCaen Cedex 5France
  4. 4.UMR 6282 CNRS “Biogéosciences”Université de Bourgogne Franche-Comté (UBFC)DijonFrance
  5. 5.Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules (ERRMECe), EA 1391, Institut des MatériauxUniversité de Cergy-PontoiseCergy-Pontoise CedexFrance
  6. 6.Department of Plastic and Reconstructive SurgerySt-Martin ClinicCaenFrance

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