Binucleated HeLa cells are formed by cytokinesis failure in starvation and keep the potential of proliferation
- 262 Downloads
Many cytological studies have reported that the numbers of binucleated cells were elevated in various tumors. However, binucleated cells are observed in not only malignant tumors but also normal tissues. Thus, the clinical significance of binucleated cells is controversial. Here we attempted to elucidate the characteristics of binucleated HeLa cells using time-lapse microscopy. To examine the frequency, viability, proliferation, and formation mechanism of binucleated cells, we grew HeLa cells on chamber slides and tissue culture dishes in DMEM supplemented with (10, 3, 1 and 0.5 % media) and without fetal bovine serum (0 % medium). The proliferation was evaluated by the medium improvement examination (cultured for 2 more days in 10% medium after culturing in 0% medium; starvation). In the 0 % medium, 150 binucleated cells were formed by cytokinesis failure. There were significantly more binucleated cells in the 0 % medium than in the 10, 3, 1 and 0.5 % media. About twice the number of binucleated cells underwent mitosis in the improvement examinations than in the serum-free examination. We found here that starvation induced the binucleation of HeLa cells and that some binucleated cells can reproduce. These findings might be helpful for understanding binucleated cells in tumors.
KeywordsBinucleated cell Formation mechanism Proliferation Time-lapse
The authors would like to thank Kaori Furuya and Ayako Ogino for their technical assistance.
Conflict of interest
The authors declare no conflicts of interest.
- Bollmann M, Bánkfalvi A, Trosic A, Speich N, Schmittt C, Bollmann R (2005) Can we detect cervical human papillomavirus (HPV) infection by cytomorphology alone? Diagnostic value of non-classic cytological signs of HPV effect in minimally abnormal Pap tests. Cytopathology 16:13–21CrossRefGoogle Scholar
- Mizoguchi K, Ishikawa K (1994) Medical technology—color atlas and histopathology. Ishiyaku, TokyoGoogle Scholar
- Papanicolaou GN (1942) A new procedure for staining vaginal smears. Science 95:432Google Scholar
- Polunovsky VA, Ingbar DH, Bitterman PB (1996) Cell fusion to study nuclear-cytoplasmic interactions in endothelial cell apoptosis. Am J Pathol 149:115–128Google Scholar
- Rinaldo C, Moncada A, Gradi A, Ciuffini L, D’Eliseo D, Siepi F, Prodosmo A, Giorgi A, Pierantoni GM, Trapasso F, Guarguaglini G, Bartolazzi A, Cundari E, Schininà ME, Fusco A, Soddu S (2012) HIPK2 controls cytokinesis and prevents tetraploidization by phosphorylating histone H2B at the midbody. Mol Cell 47:87–98CrossRefGoogle Scholar
- Zeng ZY, Chen JM (2009) Cell–cell fusion: human multinucleated osteoclasts. Cent Eur J Biol 4:543–548Google Scholar