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Cytogenetic and oxidative alterations after exposure of cultured human whole blood cells to lithium metaborate dehydrate

Cytotechnology volume 68, pages 821–827 (2016)Cite this article

Abstract

Boron compounds have an ability of supporting antioxidant properties in human and animal tissues. Lithium metaborate dihydrate (LiBO2·2H2O; LMD) is commonly used in nonlinear optic materials, cellular phones and pagers. But, there are limited data on the genotoxic and antioxidant effects of LMD in cultured human whole blood cells. The aim of this study was to evaluate for the genotoxicity and antioxidant/oxidant activity of LMD on human whole blood lymphocytes (n = 5). LMD was applied at various concentrations (0–1,280 µg/ml) to cultured blood samples. Antioxidant/oxidant activity was evaluated by measuring the total oxidant status (TOS) and total antioxidant capacity levels. Micronuclei and chromosomal aberration tests were used in genotoxicity studies. Our results clearly revealed that all tested concentrations of LMD were found to be non-genotoxic when compared to that of the control group. In addition, LMD exhibited antioxidant activities at low concentrations. In addition the TOS levels were not changed at all concentrations of LMD. Consequently, our results clearly demonstrated that LMD is non-genotoxic and it has an important antioxidant potential in vitro.

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Affiliations

  1. Department of Chemistry, Science and Letter Faculty, Bitlis Eren University, 1300, Bitlis, Turkey

    Fatih Çağlar Çelikezen & Mehmet Sait İzgi

  2. Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey

    Başak Toğar

  3. Department of Biochemistry, Faculty of Medicine, Atatürk University, Erzurum, Turkey

    Fatma Betül Özgeriş

  4. Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey

    Hasan Türkez

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  1. Fatih Çağlar Çelikezen
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  2. Başak Toğar
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Correspondence to Fatih Çağlar Çelikezen.

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Çelikezen, F.Ç., Toğar, B., Özgeriş, F.B. et al. Cytogenetic and oxidative alterations after exposure of cultured human whole blood cells to lithium metaborate dehydrate. Cytotechnology 68, 821–827 (2016). https://doi.org/10.1007/s10616-014-9833-x

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  • DOI: https://doi.org/10.1007/s10616-014-9833-x

Keywords

  • Lithium metaborate dihydrate
  • Total antioxidant capacity
  • Total oxidant status
  • Micronuclei
  • Chromosomal aberration