, Volume 68, Issue 4, pp 771–781 | Cite as

Autologous transplantation of CD34+ bone marrow derived mononuclear cells in management of non-reconstructable critical lower limb ischemia

  • Ahmed M. Ismail
  • Said M. Abdou
  • Hassan Abdel Aty
  • Adel H. Kamhawy
  • Mohammed Elhinedy
  • Mohammed Elwageh
  • Atef Taha
  • Amal Ezzat
  • Hoda A. Salem
  • Said Youssif
  • Mohamed L. SalemEmail author
Original Research


Patients with a decrease in limb perfusion with a potential threat to limb viability manifested by ischemic rest pain, ischemic ulcers, and/or gangrene are considered to have critical limb ischemia (CLI). Because of this generally poor outcome, there is a strong need for attempting any procedure to save the affected limb. The aim of this work is to evaluate the possibility to use stem cell therapy as a treatment option for patients with chronic critical lower limb ischemia with no distal run off. This study includes 20 patients with chronic critical lower limb ischemia with no distal run off who are unsuitable for vascular or endovascular option. These patients underwent stem cell therapy (SCT) by autologous transplantation of bone marrow derived mononuclear cells. 55 % of patients treated with SCT showed improvement of the rest pain after the first month, 60 % continued improvement of the rest pain after 6 months, 75 % after 1 year and 80 % after 2 years and continued without any deterioration till the third year. Limb salvage rate after STC was 80 % after the first year till the end of the second and third years. SCT can result in angiogenesis in patients with no-option CLI, providing a foundation for the application of this therapy to leg ischemia.


Bone marrow G-CSF Leg ischemia Mobilization CD34+ cells 



This work was funded by a grant from the Research Development Fund, Tanta University, Egypt.

Conflict of interest

The authors indicate no potential conflicts of interest.


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Ahmed M. Ismail
    • 1
  • Said M. Abdou
    • 2
  • Hassan Abdel Aty
    • 1
  • Adel H. Kamhawy
    • 1
  • Mohammed Elhinedy
    • 1
  • Mohammed Elwageh
    • 1
  • Atef Taha
    • 3
  • Amal Ezzat
    • 2
  • Hoda A. Salem
    • 4
  • Said Youssif
    • 5
  • Mohamed L. Salem
    • 6
    Email author
  1. 1.Vascular Surgery UnitTanta UniversityTantaEgypt
  2. 2.Clinical Pathology DepartmentTanta UniversityTantaEgypt
  3. 3.Internal Medicine Department, Faculty of MedicineTanta UniversityTantaEgypt
  4. 4.Faculty of PharmacyAl-Azhar UniversityCairoEgypt
  5. 5.Faculty of MedicineAin Shams UniversityCairoEgypt
  6. 6.Immunology and Biotechnology Unit, Zoology Department, Faculty of Science, Center of Excellence in Cancer ResearchTanta UniversityTantaEgypt

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