, Volume 68, Issue 4, pp 763–770 | Cite as

Cardiac cell proliferation assessed by EdU, a novel analysis of cardiac regeneration

  • Bin ZengEmail author
  • Suiyang Tong
  • Xiaofeng Ren
  • Hao XiaEmail author
Original Research


Emerging evidence suggests that mammalian hearts maintain the capacity for cardiac regeneration. Rapid and sensitive identification of cardiac cellular proliferation is prerequisite for understanding the underlying mechanisms and strategies of cardiac regeneration. The following immunologically related markers of cardiac cells were analyzed: cardiac transcription factors Nkx2.5 and Gata 4; specific marker of cardiomyocytes TnT; endothelial cell marker CD31; vascular smooth muscle marker smooth muscle myosin IgG; cardiac resident stem cells markers IsL1, Tbx18, and Wt1. Markers were co-localized in cardiac tissues of embryonic, neonatal, adult, and pathological samples by 5-ethynyl-2′-deoxyuridine (EdU) staining. EdU was also used to label isolated neonatal cardiomyocytes in vitro. EdU robustly labeled proliferating cells in vitro and in vivo, co-immunostaining with different cardiac cells markers. EdU can rapidly and sensitively label proliferating cardiac cells in developmental and pathological states. Cardiac cell proliferation assessed by EdU is a novel analytical tool for investigating the mechanism and strategies of cardiac regeneration in response to injury.


Cardiac cells Proliferation EdU Regeneration Injury 



This work was supported by the Chinese National Nature Science Foundation (30900609, 81270271).

Conflict of interest

The authors do not have any possible conflicts of interest.

Supplementary material

10616_2014_9827_MOESM1_ESM.ppt (1000 kb)
Supplementary Fig. 1: EdU-labeled cardiac proliferating cells in adult hearts at E10.5, E17.5, and neonatal mice at 7 days (×40). The highest red fluorescence in the atrial and ventricular walls and ventricular septum (VS) in the fetus; staining was noticeably reduced after birth (PPT 1000 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Department of CardiologyRenmin Hospital of Wuhan UniversityWuhanPeople’s Republic of China
  2. 2.Department of Developmental and Regenerative BiologyMount sinai school of medicineNew YorkUSA
  3. 3.College of Veterinary MedicineNortheast Agricultural UniversityHarbinPeople’s Republic of China

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