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Cytotechnology

, Volume 68, Issue 2, pp 261–266 | Cite as

Evaluation of maternal and embryotoxic effects following the treatment of chloral hydrate in Drosophila melanogaster

  • Arif Ayar
  • Deniz Altun Çolak
  • Handan Uysal
Original Research

Abstract

Chloral hydrate (CH) is commonly used as a sedative and a hypnotic in pediatric medicine. In this study, the effects of CH on various developmental stages of Drosophila melanogaster were investigated. Different concentrations of CH (0.1; 0.3; 0.5 and 1 mg/mL) were used during development of the flies. Maternal toxicity due to increasing the concentration of CH was observed as a large number of adult flies died. When the F1 progeny of the control and application groups were compared, CH was found to extend the process of metamorphosis and to decrease the total number of offspring. The embryotoxic effects on the offspring and an increase in the number of malformed offspring was identified as depending on feeding. It was found that the difference between the groups was significantly important (p < 0.05).

Keywords

Drosophila melanogaster Chloral hydrate Developmental stages Maternal toxicity Embryotoxic effect 

Notes

Conflict of interest

The Authors declare that there is no conflict of interest.

References

  1. Altun D, Uysal H, Ayar A, Aşkın H (2011) Removal of the toxic effects of chlormadinon acetate on the development of Drosophila melanogaster via the use of nordihydroguaiaretic acid. Toxicol Ind Health 27:29–33CrossRefGoogle Scholar
  2. Baselt RC (2004) Disposition of toxic drugs and chemicals in man, 7th edn. Biomedical Publications, Foster City, pp 187–190Google Scholar
  3. Bowyer K, Glasser P (1980) Chloral hydrate overdose and cardiac arrhythmias. Chest 77:232–235CrossRefGoogle Scholar
  4. Bringmann G, God R, Fähr S, Feineis D, Fornadi K, Fornadi F (1999) Identification of the dopaminergic neurotoxin 1-trichloromethyl-1,2,3,4-tetrahydro-beta-carboline in human blood after intake of the hypnotic chloral hydrate. Anal Biochem 270:167–175Google Scholar
  5. Britton JW, Kosa SC (2010) The clinical value of chloral hydrate in the routine electroencephalogram. Epilepsy Res 88:215–220CrossRefGoogle Scholar
  6. Daniel FB, DeAngelo AB, Stober JA, Olson GR, Page NP (1992) Hepatocarcinogenicity of chloral hydrate, 2-chloroacetaldehyde, and dichloroacetic acid in the male B6C3F1 mouse. Fundam Appl Toxicol 19:159–168CrossRefGoogle Scholar
  7. Dogan-Duyar S, Willemse JL, Van Hee P, Duval EL, Neels H (2010) Chloral hydrate intoxication in a 3-month-old child: avoidance of haemodialysis by an immediate determination of trichloroethanol. Clin Biochem 43:328–330CrossRefGoogle Scholar
  8. Englehart DA, Lavins ES, Hazenstab CB, Sutheimer CA (1998) Unusual death attributed to the combined effects of chloral hydrate, lidocaine, and nitrous oxide. J Anal Toxicol 22:246–247CrossRefGoogle Scholar
  9. Gerretsen M, de Groot G, van Heijst AN, Maes RA (1979) Chloral hydrate poisoning: its mechanism and therapy. Vet Hum Toxicol 21:53–56Google Scholar
  10. Graham SR, Day RO, Lee R, Fulde GW (1988) Overdose with chloral hydrate: a pharmacological and therapeutic review. Med J Aust 149:686–688Google Scholar
  11. Green T, Mainwaring GW, Foster JR (1997) Trichloroethylene-induced mouse lung tumours: studies of the mode of action and comparisons between species. Fundam Appl Toxicol 37:125–130CrossRefGoogle Scholar
  12. Kappas A (1989) On the mechanisms of induced aneuploidy in Aspergillus nidulans and validation of tests for genomic mutations. Prog Clin Biol Res 318:377–384Google Scholar
  13. Kost S, Roy A (2010) Procedural sedation and analgesia in the pediatric emergency department: a review of sedative pharmacology. Clin Ped Emerg Med 11:233–243CrossRefGoogle Scholar
  14. Laurent Y, Wallemacq P, Haufroid V (2006) Electrocardiographic changes with segmental akinesia after chloral hydrate overdose. Int J Emerg Med 30:179–182CrossRefGoogle Scholar
  15. Lin YC, Ma J (2006) Severe oesophageal burn following chloral hydrate overdose in an infant. J Formos Med Assoc 105:235–237CrossRefGoogle Scholar
  16. Lipscomb JC, Mahle DA, Brashear WT, Garrett CM (1996) A species comparison of chloral hydrate metabolism in blood and liver. Biochem Biophys Res Commun 227:340–350CrossRefGoogle Scholar
  17. Lu J, Greco MA (2006) Sleep circuitry and the hypnotic mechanism of GABAA drugs. J Clin Sleep Med 2:19–26Google Scholar
  18. Ludwigs U, Divino Filho JC, Magnusson A, Berg A (1996) Suicidal chloral hydrate poisoning. J Toxicol Clin Toxicol 34:97–99CrossRefGoogle Scholar
  19. Lynch DW, Schuler RL, Hood RD, Davis DG (1991) Evaluation of Drosophila for screening developmental toxicants: test results with eighteen chemicals and presentation of a new Drosophila bioassay. Teratog Carcinog Mutagen 11:147–173CrossRefGoogle Scholar
  20. Mason JM, Valencia R, Zimmering S (1992) Chemical mutagenesis testing in Drosophila: VIII. Re-examination of equivocal results. Environ Mol Mutagen 19:227–234CrossRefGoogle Scholar
  21. Merdink JL, Robison LM, Stevens DK, Hu M, Parker JC, Bull RJ (2008) Kinetics of chloral hydrate and its metabolites in male human volunteers. Toxicology 245:130–140Google Scholar
  22. Ni YC, Wong TY, Kadlubar FF, Fu PP (1994) Hepatic metabolism of chloral hydrate to free radical(s) and induction of lipid peroxidation. Biochem Biophys Res Commun 204:937–943CrossRefGoogle Scholar
  23. Ni YC, Wong TY, Lloyd RV, Heinze TM, Shelton S, Casciano D, Kadlubar FF, Fu PP (1996) Mouse liver microsomal metabolism of chloral hydrate, trichloroacetic acid, and trichloroethanol leading to induction of lipid peroxidation via a free radical mechanism. Drug Metab Dispos 24:81–90Google Scholar
  24. Pereira MA (1996) Carcinogenic activity of dichloroacetic acid and trichloroacetic acid in the liver of female B6C3F1 mice. Fundam Appl Toxicol 31:192–199CrossRefGoogle Scholar
  25. Petersen NS (1990) Effects of heat and chemical stress on development. Advan Gene 28:275–296CrossRefGoogle Scholar
  26. Rand MD (2010) Drosophotoxicology: the growing potential for Drosophila in neurotoxicology. Neurotoxicol Teratol 32:74–83CrossRefGoogle Scholar
  27. Rodrigues F, Schmidt I, Klambt C (2011) Comparing peripheral glial cell differentiation in Drosophila and vertebrates. Cell Mol Life Sci 68:55–69CrossRefGoogle Scholar
  28. Rokicki W (1996) Cardiac arrhythmia in a child after the usual dose of chloral hydrate. Pediatr Cardiol 17:419–420CrossRefGoogle Scholar
  29. Sanders VM, Kauffman BM, White KL Jr, Douglas KA, Barnes DW, Sain LE, Bradshaw TJ, Borzelleca JF, Munson AE (1982) Toxicology of chloral hydrate in the mouse. Environ Health Perspect 44:137–146CrossRefGoogle Scholar
  30. Schatten H, Chakrabarti A (1998) Centrosome structure and function is altered by chloral hydrate and diazepam during the first reproductive cell cycles in sea urchin eggs. Euro J Cell Biol 75:9–20CrossRefGoogle Scholar
  31. Schuler RL, Hardin BD, Niemeier RW (1982) Drosophila as a tool for the rapid assessment of chemicals for teratogenicity. Teratog Carcinog Mutagen 2:293–301CrossRefGoogle Scholar
  32. Sora S, Agostini Carbone ML (1987) Chloral hydrate, methylmercury hydroxide and ethidium bromide affect chromosomal segregation during meiosis of Saccharomyces cerevisiae. Mutat Res 190:13–17CrossRefGoogle Scholar
  33. Uysal H, Aşkın H (2007) The effects of chronic phenol exposure via diet on developmental stages of Drosophila melanogaster (Diptera:Drosophilidae). Fres Environ Bull 16:991–997Google Scholar
  34. Vian L, Van Hummelen P, Bichet N, Gouy D, Kirsch-Volders M (1995) Evaluation of hydroquinone and chloral hydrate on the in vitro micronucleus test on isolated lymphocytes. Mutat Res 334:1–7CrossRefGoogle Scholar
  35. Von Tungeln LS, Yi P, Bucci TJ, Samokyszyn VM, Chou MW, Kadlubar FF, Fu PP (2002) Tumorigenicity of chloral hydrate, trichloroacetic acid, trichloroethanol, malondialdehyde, 4-hydroxy-2-nonenal, crotonaldehyde, and acrolein in the B6C3F(1) neonatal mouse. Cancer Lett 185:13–19Google Scholar
  36. Wong OF, Lam TSK, Fung HT (2009) Two cases of chloral hydrate overdose. Hong Kong J Emerg Med 16:161–167Google Scholar
  37. Zahedi A, Grant M, Wong D (1999) Successful treatment of chloral hydrate cardiac toxicity with propranolol. Am J Emerg Med 17:490–491CrossRefGoogle Scholar
  38. Zordan M, Osti M, Pesce M, Costa R (1994) Chloral hydrate is recombinogenic in the wing spot test in Drosophila melanogaster. Mutat Res 322:111–116CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Sabuncuoğlu Şerefeddin Health Services Vocational SchoolAmasya UniversityAmasyaTurkey
  2. 2.Department of Biology, Faculty of Art and ScienceErzincan UniversityErzincanTurkey
  3. 3.Department of Biology, Faculty of ScienceAtatürk UniversityErzurumTurkey

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