Derivation of a novel G2 reporter system
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Progression through G2 phase of the cell cycle is a technically difficult area of cell biology to study due to the lack of physical markers specific to this phase. The FUCCI system uses the biology of the cell cycle to drive fluorescence in select phases of the cell cycle. Similarly, a commercially available system has used a fluorescent analog of the Cyclin B1 protein to visualize cells from late S phase to the metaphase–anaphase transition. We have modified these systems to use the promoter and destruction box elements of Cyclin B1 to drive a cyan fluorescent protein. We demonstrate here that this is a useful tool for measuring the length of G2 phase without perturbing any aspect of cell cycle progression.
KeywordsCell cycle G2 phase Mitosis DNA synthesis Cyclin B1
We thank Bob Hodge and Jiamila Maimaiti for technical assistance. We also thank Dorota Lubanska and Kaitlyn Matthews for feedback on this work. S.B. acknowledges support from the Natural Science and Engineering Research Council of Canada (NSERC) Undergraduate Student Research Assistantship Program (USRA). This work was supported by an NSERC grant (#312014-2009).
Conflict of interest
The authors have no conflicts to declare.
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