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Cytotechnology

, Volume 67, Issue 1, pp 157–163 | Cite as

Genotoxicity of indium tin oxide by Allium and Comet tests

  • İbrahim Hakkı Ciğerci
  • Recep LimanEmail author
  • Emre Özgül
  • Muhsin Konuk
Original Research

Abstract

Genotoxic effects of indium tin oxide (ITO) were investigated on root cells of Allium cepa by employing both Allium and Comet assays. A. cepa roots were treated with the aqueous dispersions of ITO at 5 different concentrations (12.5, 25, 50, 75, and 100 ppm) for 4 h. Exposure of ITO significantly increased mitotic index, and total chromosomal aberrations by the Allium test. While chromosome laggards, stickiness, disturbed anaphase–telophase and anaphase bridges were observed in anaphase–telophase cells, c-metaphase and binuclear cells were observed in other cells. A significant increase in DNA damage was also observed at all concentrations of ITO by the Comet assay. These results indicate that ITO exhibits genotoxic activity in A. cepa root meristematic cells.

Keywords

Allium test Chromosome aberration Comet assay DNA damage 

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • İbrahim Hakkı Ciğerci
    • 1
  • Recep Liman
    • 2
    Email author
  • Emre Özgül
    • 1
  • Muhsin Konuk
    • 3
  1. 1.Biology Department, Faculty of Science and LiteraturesAfyon Kocatepe UniversityAfyonTurkey
  2. 2.Molecular Biology and Genetics Department, Faculty of Arts and SciencesUşak UniversityUşakTurkey
  3. 3.Molecular Biology and Genetics Department, Faculty of Engineering and Natural SciencesÜsküdar UniversityIstanbulTurkey

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