The major polyphenols in green tea, (−)-epigallocatechin and (−)-epigallocatechin gallate, have been shown to enhance the phagocytic activity of macrophage-like cells; however, the mechanism involved was not clarified. In this study, we have identified that the catechins induced phagocytosis can be blocked by catalase and an inhibitor of transient receptor potential melastatin 2.
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Arakawa H, Maeda M, Okubo S, Shimamura T (2004) Role of hydrogen peroxide in bactericidal action of catechin. Biol Pharm Bull 27:277–281
Azam S, Hadi N, Khan NU, Hadi SM (2004) Prooxidant property of green tea polyphenols epicatechin and epigallocatechin-3-gallate: implications for anticancer properties. Toxicol In Vitro 18:555–561
Di A, Gao XP, Qian F, Kawamura T, Han J, Hecquet C, Ye RD, Vogel SM, Malik AB (2011) The redox-sensitive cation channel TRPM2 modulates phagocyte ROS production and inflammation. Nat Immunol 13:29–34
Fujimura Y, Umeda D, Yano S, Maeda-Yamamoto M, Yamada K, Tachibana H (2007) The 67 kDa laminin receptor as a primary determinant of anti-allergic effects of O-methylated EGCG. Biochem Biophys Res Commun 364:79–85
Furukawa A, Oikawa S, Murata M, Hiraku Y, Kawanishi S (2003) (−)-Epigallocatechin gallate causes oxidative damage to isolated and cellular DNA. Biochem Pharmacol 66:1769–1778
Gardella S, Andrei C, Poggi A, Zocchi MR, Rubartelli A (2000) Control of interleukin-18 secretion by dendritic cells: role of calcium influxes. FEBS Lett 481:245–248
Gardella S, Andrei C, Lotti LV, Poggi A, Torrisi MR, Zocchi MR, Rubartelli A (2001) CD8(+) T lymphocytes induce polarized exocytosis of secretory lysosomes by dendritic cells with release of interleukin-1beta and cathepsin D. Blood 98:2152–2159
Kashio M, Sokabe T, Shintaku K, Uematsu T, Fukuta N, Kobayashi N, Mori Y, Tominaga M (2012) Redox signal-mediated sensitization of transient receptor potential melastatin 2 (TRPM2) to temperature affects macrophage functions. Proc Natl Acad Sci U S A 109:6745–6750
Matoba T, Shimokawa H, Nakashima M, Hirakawa Y, Mukai Y, Hirano K, Kanaide H, Takeshita A (2000) Hydrogen peroxide is an endothelium-derived hyperpolarizing factor in mice. J Clin Invest 106:1521–1530
Monobe M, Ema K, Kato F, Hirokane H, Maeda-Yamamoto M (2007) Technique for screening immune-enhancing polysaccharides in food using 1,25-dihydroxyvitamin D3-differentiated HL60 cells. J Agric Food Chem 55:2543–2547
Monobe M, Ema K, Tokuda Y, Maeda-Yamamoto M (2010) Enhancement of phagocytic activity of macrophage-like cells by pyrogallol-type green tea polyphenols through caspase signaling pathways. Cytotechnology 62:201–203
Oikawa S, Furukawaa A, Asada H, Hirakawa K, Kawanishi S (2003) Catechins induce oxidative damage to cellular and isolated DNA through the generation of reactive oxygen species. Free Radic Res 37:881–890
Sugisawa A, Umegaki K (2002) Physiological concentrations of (−)-epigallocatechin-3-O-gallate (EGCg) prevent chromosomal damage induced by reactive oxygen species in WIL2-NS cells. J Nutr 132:1836–1839
Tachibana H, Koga K, Fujimura Y, Yamada K (2004) A receptor for green tea polyphenol EGCG. Nat Struct Mol Biol 11:380–381
Vanhoutte PM (2001) Endothelium-derived free radicals: for worse and for better. J Clin Invest 107:23–25
Wehrhahn J, Kraft R, Harteneck C, Hauschildt S (2010) Transient receptor potential melastatin 2 is required for lipopolysaccharide-induced cytokine production in human monocytes. J Immunol 184:2386–2393
Yamamoto S, Shimizu S, Kiyonaka S, Takahashi N, Wajima T, Hara Y, Negoro T, Hiroi T, Kiuchi Y, Okada T, Kaneko S, Lange I, Fleig A, Penner R, Nishi M, Takeshima H, Mori Y (2008) TRPM2-mediated Ca2 + influx induces chemokine production in monocytes that aggravates inflammatory neutrophil infiltration. Nat Med 14:738–747
This work was supported by a grant for project research (Development of fundamental technology for analysis and evaluation of functional agricultural products and functional foods), Ministry of Agriculture, Fishery and Forestry, Japan.
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Monobe, M., Ema, K., Tokuda, Y. et al. Green tea catechin induced phagocytosis can be blocked by catalase and an inhibitor of transient receptor potential melastatin 2 (TRPM2). Cytotechnology 66, 561–566 (2014). https://doi.org/10.1007/s10616-013-9618-7
- (−)-Epigallocatechin (EGC)
- (−)-Epigallocatechin gallate (EGCG)
- Hydrogen peroxide