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Chemoprotective activity of the isoflavones, genistein and daidzein on mutagenicity induced by direct and indirect mutagens in cultured HTC cells

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Abstract

Isoflavones are phenolic compounds widely distributed in plants and found in a high percentage in soybeans. They have important biological properties and are regarded as potential chemopreventive agents. The aim of this study was to verify the preventive effect of two soy isoflavones (genistein and daidzein) by a micronucleus assay, analysis of GST activity, and real-time RT-PCR analysis of GSTa2 gene expression. Mutagens of direct (doxorubicin) and indirect (2-aminoanthracene) DNA damage were used. Hepatoma cells (HTC) were treated with genistein or daidzein for 26 h at noncytotoxic concentrations; 10 μM when alone, and 0.1, 1.0 and 10 μM when combined with genotoxic agents. The micronucleus test demonstrated that both isoflavones alone had no genotoxic effect. Genistein showed antimutagenic effects at 10 μM with both direct and indirect DNA damage agents. On phase II enzyme regulation, the current study indicated an increase in total cytoplasmic GST activity in response to genistein and daidzein at 10 μM supplementation. However, the mRNA levels of GSTa2 isozymes were not differentially modulated by genistein or daidzein. The results point to an in vitro antimutagenic activity of genistein against direct and indirect DNA damage-induced mutagenicity.

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Acknowledgments

The authors thank National Council of Scientific and Technological Development (CNPq), Coordination for the Improvement of Superior Level (CAPES) and Araucaria Foundation for the Support of Scientific and Technological Development of Parana—Brazil.

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The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. General Biology Department, State University of Londrina (UEL), Rodovia Celso Garcia Cid, Pr 445 km 380, Campus Universitário, Cx. Postal 6001, Londrina, PR, CEP 86051-980, Brazil

    Sandra Regina Lepri, Leonardo Campos Zanelatto, Patrícia Benites Gonçalves da Silva, Daniele Sartori & Mario Sergio Mantovani

  2. Pathological Sciences Department, State University of Londrina (UEL), Londrina, PR, Brazil

    Rodrigo Cabral Luiz

  3. Institute of Biosciences, UNESP, Rio Claro, SP, Brazil

    Lucia Regina Ribeiro

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  1. Sandra Regina Lepri
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Correspondence to Sandra Regina Lepri.

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Lepri, S.R., Luiz, R.C., Zanelatto, L.C. et al. Chemoprotective activity of the isoflavones, genistein and daidzein on mutagenicity induced by direct and indirect mutagens in cultured HTC cells. Cytotechnology 65, 213–222 (2013). https://doi.org/10.1007/s10616-012-9476-8

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