Skip to main content

Advertisement

SpringerLink
Log in

Evaluation of immunostimulatory effect of the arrowroot (Maranta arundinacea. L) in vitro and in vivo

  • Brief Report
  • Published:
Cytotechnology Aims and scope Submit manuscript

Abstract

Arrowroot (Maranta arundinacea. L) is an underutilized local crop potentially to be developed as carbohydrate source and functional food in Indonesia. The objectives of this research are to evaluate the immunostimulatory effects of arrowroot extracts in vitro by using animal cell culture techniques, and in vivo by using BALB/c mice. The arrowroot tuber extracts were prepared by heat-treatment at 121 °C for 20 min in distilled water. The IgM production stimulatory activity of arrowroot tuber extracts against human hybridoma HB4C5 cells and mouse splenocytes was assessed. The result indicated that the arrowroot tuber extract stimulated IgM production by HB4C5 cells and immunoglobulin (IgG, IgA and IgM) production by splenocytes in vitro. In addition, the arrowroot tuber extracts strongly enhanced interferon γ production by splenocytes. In vivo study indicated that the diet containing arrowroot extracts increased the serum IgG, IgA and IgM levels in mice. These results revealed that the arrowroot tuber extracts have immunostimulatory effects in vivo as well as in vitro.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Abbreviations

Con A:

Concanavalin A

ELISA:

Enzyme-linked immunosorbent assay

FBS:

Fetal bovine serum

GALT:

Gut-associated lymphoid tissue

HRP:

Horseradish peroxidase

IFN-γ:

Interferon-γ

Ig:

Immunoglobulin

OF:

Oligofructose

PBS:

Phosphate-buffered saline

RS:

Retrograded starch

SCFA:

Short chain fatty acid

References

  • Delzenne NM, Roberfroid MB (1994) Physiological effects of non-digestible oligosaccharides. Lebensm Wiss Technol 27:1–6

    Article  CAS  Google Scholar 

  • Gibson GR, Roberfroid MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 125:1401–1412

    CAS  Google Scholar 

  • Haralampu SG (2000) Resistant starch: a review of the physical properties and biological impact of RS3. Carbohydr Polym 41:285–292

    Article  CAS  Google Scholar 

  • Harmayani E, Kumalasari ID, Marsono Y (2011) Effect of arrowroot (Maranta arundinacea L.) diet on the selected bacterial population and chemical properties of caecal digesta of Sprague Dawley rats. Int Res J Microbiol 2:278–284

    Google Scholar 

  • Jenkins DJ, Kendall CW, Vuksan V, Augustin LS, Li YM, Lee B, Mehling CC, Parker T, Faulkner D, Seyler H, Vidgen E, Fulgoni V (1999) The effect of wheat bran particle size on laxation and colonic fermentation. J Am Coll Nutr 18:339–345

    CAS  Google Scholar 

  • LeBlanc JG, Silvestroni A, Connes C, Juillard V, de Giori GS, Piard JC, Sesma F (2004) Reduction of non-digestible oligosaccharides in soymilk: application of engineered lactic acid bacteria that produce alpha-galactosidase. Genet Mol Res 3:432–440

    CAS  Google Scholar 

  • Louis P, Scott KP, Duncan SH, Flint HJ (2007) Understanding the effects of diet on bacterial metabolism in the large intestine. J Appl Microbiol 102:1197–1208

    Article  CAS  Google Scholar 

  • Murakami H, Masui H, Sato GH, Sueoka N, Chow TP, Kono-Sueoka T (1982) Growth of hybridoma cells in serum-free medium. Proc Natl Acad Sci USA 79:1158–1162

    Article  CAS  Google Scholar 

  • Murakami H, Hashizume S, Ohashi H, Shinohara K, Yasumoto K, Nomoto K, Omura H (1985) Human-human hybridomas secreting antibodies specific to human lung carcinoma. In Vitro Cell Dev Biol 21:593–596

    Article  CAS  Google Scholar 

  • Nakamura Y, Nosaka S, Suzuki M, Nagafuchi S, Takahashi T, Yajima T, Takenouchi-Ohkubo N (2004) Dietary fructooligosaccharides up-regulate immunoglobulin A response and polymeric immunoglobulin receptor expression in intestines of infant mice. Clin Exp Immunol 137:52–58

    Article  CAS  Google Scholar 

  • Nishimoto S, Kanda K, Yamawaki M, Okabe M, Akiyama K, Kakinuma Y, Sugahara T (2009) Heavy oil fractions induce the negative influences on mouse immune system. J Toxicol Sci 34:459–468

    Article  CAS  Google Scholar 

  • Reeves PG, Neilsen FH, Fahey GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951

    CAS  Google Scholar 

  • Schley PD, Field CJ (2002) The immune-enhancing effects of dietary fibers and prebiotics. Br J Nutr 87:S221–S230

    Article  CAS  Google Scholar 

  • Seifert S, Watzl B (2007) Inulin and oligofructose: review of experimental data on immune modulation. J Nutr 137:2563S–2567S

    CAS  Google Scholar 

  • Sugahara T, Onda H, Shinohara Y, Horii M, Akiyama K, Nakamoto K, Hara K (2005) Immunostimulation effects of proteose-peptone component 3 fragment on human hybridomas and peripheral blood lymphocytes. Biochim Biophys Acta 1725:233–240

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan

    Ika Dyah Kumalasari, Kosuke Nishi & Takuya Sugahara

  2. Faculty of Agricultural Technology, Gadjah Mada University, Jl. Socio Yusticia, Bulaksumur, Yogyakarta, 55281, Indonesia

    Eni Harmayani & Sri Raharjo

  3. Faculty of Medicine, Gadjah Mada University, Jl. Farmako, Sekip Utara, Yogyakarta, 55281, Indonesia

    Lily Arsanti Lestari

  4. Faculty of Veterinary Medicine, Gadjah Mada University, Jl. Fauna No. 2, Karang Malang, Yogyakarta, 55281, Indonesia

    Widya Asmara

  5. South Ehime Fisheries Research Center, Ehime University, 1289-1 Funakoshi, Ainan, Ehime, 789-4292, Japan

    Takuya Sugahara

Authors
  1. Ika Dyah Kumalasari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eni Harmayani
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lily Arsanti Lestari
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sri Raharjo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Widya Asmara
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kosuke Nishi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Takuya Sugahara
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Eni Harmayani or Takuya Sugahara.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kumalasari, I.D., Harmayani, E., Lestari, L.A. et al. Evaluation of immunostimulatory effect of the arrowroot (Maranta arundinacea. L) in vitro and in vivo. Cytotechnology 64, 131–137 (2012). https://doi.org/10.1007/s10616-011-9403-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10616-011-9403-4

Keywords

Search

Navigation