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Evaluation of immunostimulatory effect of the arrowroot (Maranta arundinacea. L) in vitro and in vivo


Arrowroot (Maranta arundinacea. L) is an underutilized local crop potentially to be developed as carbohydrate source and functional food in Indonesia. The objectives of this research are to evaluate the immunostimulatory effects of arrowroot extracts in vitro by using animal cell culture techniques, and in vivo by using BALB/c mice. The arrowroot tuber extracts were prepared by heat-treatment at 121 °C for 20 min in distilled water. The IgM production stimulatory activity of arrowroot tuber extracts against human hybridoma HB4C5 cells and mouse splenocytes was assessed. The result indicated that the arrowroot tuber extract stimulated IgM production by HB4C5 cells and immunoglobulin (IgG, IgA and IgM) production by splenocytes in vitro. In addition, the arrowroot tuber extracts strongly enhanced interferon γ production by splenocytes. In vivo study indicated that the diet containing arrowroot extracts increased the serum IgG, IgA and IgM levels in mice. These results revealed that the arrowroot tuber extracts have immunostimulatory effects in vivo as well as in vitro.

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Con A:

Concanavalin A


Enzyme-linked immunosorbent assay


Fetal bovine serum


Gut-associated lymphoid tissue


Horseradish peroxidase








Phosphate-buffered saline


Retrograded starch


Short chain fatty acid


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Correspondence to Eni Harmayani or Takuya Sugahara.

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Kumalasari, I.D., Harmayani, E., Lestari, L.A. et al. Evaluation of immunostimulatory effect of the arrowroot (Maranta arundinacea. L) in vitro and in vivo. Cytotechnology 64, 131–137 (2012).

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  • Arrowroot (Maranta arundinacea)
  • Immunoglobulin
  • Immunostimulatory effect
  • Interferon γ
  • Splenocytes