, Volume 63, Issue 2, pp 191–200 | Cite as

3,4,5-tri-O-caffeoylquinic acid inhibits amyloid β-mediated cellular toxicity on SH-SY5Y cells through the upregulation of PGAM1 and G3PDH

  • Yusaku Miyamae
  • Junkyu Han
  • Kazunori Sasaki
  • Mika Terakawa
  • Hiroko IsodaEmail author
  • Hideyuki ShigemoriEmail author
JAACT Special Issue


Caffeoylquinic acid (CQA) is one of the phenylpropanoids found in a variety of natural resources and foods, such as sweet potatoes, propolis, and coffee. Previously, we reported that 3,5-di-O-caffeoylquinic acid (3,5-di-CQA) has a neuroprotective effect against amyloid-β (Aβ)-induced cell death through the overexpression of glycolytic enzyme. Additionally, 3,5-di-CQA administration induced the improvement of spatial learning and memory on senescence accelerated-prone mice (SAMP8). The aim of this study was to investigate whether 3,4,5-tri-O-caffeoylquinic acid (3,4,5-tri-CQA), isolated from propolis, shows a neuroprotective effect against Aβ-induced cell death on human neuroblastoma SH-SY5Y cells. To clarify the possible mechanism, we performed proteomics and real-time RT–PCR as well as a measurement of the intracellular adenosine triphosphate (ATP) level. These results showed that 3,4,5-tri-CQA attenuated the cytotoxicity and prevented Aβ-mediated apoptosis. Glycolytic enzymes, phosphoglycerate mutase 1 (PGAM1) and glyceraldehyde-3-phosphate dehydrogenase (G3PDH) were overexpressed in co-treated cells with both 3,4,5-tri-CQA and Aβ. The mRNA expression of PGAM1, G3PDH, and phosphoglycerate kinase 1 (PGK1), and intracellular ATP level were also increased in 3,4,5-tri-CQA treated cells. Taken together the findings in our study suggests that 3,4,5-tri-CQA shows a neuroprotective effect against Aβ-induced cell death through the upregulation of glycolytic enzyme mRNA as well as ATP production activation.


3,4,5-tri-O-caffeoylquinic acid SH-SY5Y cells G3PDH PGAM1 Glycolytic enzyme ATP 


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Yusaku Miyamae
    • 1
  • Junkyu Han
    • 1
    • 2
  • Kazunori Sasaki
    • 1
  • Mika Terakawa
    • 3
  • Hiroko Isoda
    • 1
    • 2
    Email author
  • Hideyuki Shigemori
    • 1
    Email author
  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukuba, IbarakiJapan
  2. 2.Alliance for Research on North Africa (ARENA), University of TsukubaTsukuba, IbarakiJapan
  3. 3.Kato Brothers Honey Co., Ltd.Yokohama, KanagawaJapan

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