Skip to main content

Inhibitory effect of hot-water extract of quince (Cydonia oblonga) on immunoglobulin E-dependent late-phase immune reactions of mast cells

An Erratum to this article was published on 21 July 2011

An Erratum to this article was published on 21 July 2011

Abstract

We evaluated the effect of a crude hot-water extract (HW) of quince (Cydonia oblonga Miller) fruit on immunoglobulin E (IgE)-dependent late-phase immune reactions of mast cells using in vitro system. Mast cell-like RBL-2H3 cells were treated with quince HW and late-phase reaction was then induced by stimulation with IgE + Antigen. Quince HW reduced the elevation of interleukin-13 and tumor necrosis factor-α expression level. Furthermore, quince HW suppressed these cytokine expressions of mouse bone marrow-derived mast cells (BMMCs), a normal mast cell model. Leukotriene C4 and prostaglandin D2 production in BMMCs after 1 and 6 h of stimulation, respectively, were also reduced by treating the cells with quince HW. We found that the induction of intracellular cyclooxygenase (COX)-2 expression but not COX-1 expression in BMMCs was reduced by quince HW. These results suggest that quince HW has an inhibitory effect on broad range of the late-phase immune reactions of mast cells.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Abbreviations

Ag:

Antigen

BMMC:

Bone marrow-derived mast cell

COX:

Cyclooxygenase

ELISA:

Enzyme-linked immunosorbent assay

FBS:

Fetal bovine serum

FcεRI:

High-affinity IgE receptor

FITC:

Fluorescein isothiocyanate

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

HW:

Hot-water extract

IgE:

Immunoglobulin E

IL:

Interleukin

LT:

Leukotriene

NSAID:

Nonsteroidal anti-inflammatory drug

PBS:

Phosphate-buffered saline

PE:

Phycoerythrin

PG:

Prostaglandin

RT–PCR:

Reverse transcription-polymerase chain reaction

SD:

Standard deviation

TNF:

Tumor necrosis factor

References

  • Burd PR, Thompson WC, Max EE, Mills FC (1995) Activated mast cells produce interleukin 13. J Exp Med 181:1373–1380

    Article  CAS  Google Scholar 

  • Cameron L, Hamid Q, Wright E, Nakamura Y, Christodoulopoulos P, Muro S, Frenkiel S, Lavigne F, Durham S, Gould H (2000) Local synthesis of epsilon germline gene transcripts, IL-4, and IL-13 in allergic nasal mucosa after ex vivo allergen exposure. J Allergy Clin Immunol 106:46–52

    Article  CAS  Google Scholar 

  • Dahlén SE, Björk J, Hedqvist P, Arfors KE, Hammarström S, Lindgren JA, Samuelsson B (1981) Leukotrienes promote plasma leakage and leukocyte adhesion in postcapillary venules: in vivo effects with relevance to the acute inflammatory response. Proc Natl Acad Sci USA 78:3887–3891

    Article  Google Scholar 

  • Dahlén SE, Hansson G, Hedqvist P, Björck T, Granström E, Dahlén B (1983) Allergen challenge of lung tissue from asthmatics elicits bronchial contraction that correlates with the release of leukotrienes C4, D4, and E4. Proc Natl Acad Sci USA 80:1712–1716

    Article  Google Scholar 

  • Funaba M, Ikeda T, Abe M (2003) Degranulation in RBL-2H3 cells: regulation by calmodulin pathway. Cell Biol Int 27:879–885

    Article  CAS  Google Scholar 

  • Giles H, Leff P (1988) The biology and pharmacology of PGD2. Prostaglandins 35:277–300

    Article  CAS  Google Scholar 

  • Gordon JR, Galli SJ (1990) Mast cells as a source of both preformed and immunologically inducible TNF-alpha/cachectin. Nature 346:274–276

    Article  CAS  Google Scholar 

  • Gordon JR, Galli SJ (1991) Release of both preformed and newly synthesized tumor necrosis factor alpha (TNF-α)/cachectin by mouse mast cells stimulated via the FcεRI. A mechanism for the sustained action of mast cell-derived TNF-α during IgE-dependent biological responses. J Exp Med 174:103–107

    Article  CAS  Google Scholar 

  • Harirforoosh S, Jamali F (2009) Renal adverse effects of nonsteroidal anti-inflammatory drugs. Expert Opin Drug Saf 8:669–681

    Article  CAS  Google Scholar 

  • Hundley TR, Prasad AR, Beaven MA (2001) Elevated levels of cyclooxygenase-2 in antigen-stimulated mast cells is associated with minimal activation of p38 mitogen-activated protein kinase. J Immunol 167:1629–1636

    CAS  Google Scholar 

  • Kang NI, Kim HK, Ko HM, Kim JH, You HJ, Choi IW, Im SY, Lee HK (2008) Tumor necrosis factor-alpha develops late anaphylactic reaction through cytosolic phospholipase A(2) activation. Int Arch Allergy Immunol 147:315–322

    Article  CAS  Google Scholar 

  • Kawata R, Reddy ST, Wolner B, Herschman HR (1995) Prostaglandin synthase 1 and prostaglandin synthase 2 both participate in activation-induced prostaglandin D2 production in mast cells. J Immunol 155:818–825

    CAS  Google Scholar 

  • Lee E, Yook J, Haa K, Chang HW (2005) Induction of Ym1/2 in mouse bone marrow-derived mast cells by IL-4 and identification of Ym1/2 in connective tissue type-like mast cells derived from bone marrow cells cultured with IL-4 and stem cell factor. Immunol Cell Biol 83:468–474

    Article  CAS  Google Scholar 

  • Metz M, Grimbaldeston MA, Nakae S, Piliponsky AM, Tsai M, Galli SJ (2007) Mast cells in the promotion and limitation of chronic inflammation. Immunol Rev 217:304–328

    Article  CAS  Google Scholar 

  • Metzger H (1978) The IgE-mast cell system as a paradigm for the study of antibody mechanisms. Immunol Rev 41:186–199

    Article  CAS  Google Scholar 

  • Murakami M, Matsumoto R, Austen KF, Arm JP (1994) Prostaglandin endoperoxide synthase-1 and -2 couple to different transmembrane stimuli to generate prostaglandin D2 in mouse bone marrow-derived mast cells. J Biol Chem 269:22269–22275

    CAS  Google Scholar 

  • Ortega E, Schweitzer-Stenner R, Pecht I (1988) Possible orientational constraints determine secretory signals induced by aggregation of IgE receptors on mast cells. EMBO J 7:4101–4109

    CAS  Google Scholar 

  • Passante E, Ehrhardt C, Sheridan H, Frankish N (2009) RBL-2H3 cells are an imprecise model for mast cell mediator release. Inflamm Res 58:611–618

    Article  CAS  Google Scholar 

  • Pawankar R, Yamagishi S, Takizawa R, Yagi T (2003) Mast cell-IgE-and mast cell–structural cell interactions in allergic airway disease. Curr Drug Targets Inflamm Allergy 2:303–312

    Article  CAS  Google Scholar 

  • Qin HD, Shi YQ, Liu ZH, Li ZG, Wang HS, Wang H, Liu ZP (2010) Effect of chlorogenic acid on mast cell-dependent anaphylactic reaction. Int Immunopharmacol 10:1135–1141

    Article  CAS  Google Scholar 

  • Razin E, Cordon-Cardo C, Good RA (1981) Growth of a pure population of mouse mast cells in vitro with conditioned medium derived from concanavalin A-stimulated splenocytes. Proc Natl Acad Sci USA 78:2559–2561

    Article  CAS  Google Scholar 

  • Razin E, Mencia-Huerta JM, Lewis RA, Corey EJ, Austen KF (1982) Generation of leukotriene C4 from a subclass of mast cells differentiated in vitro from mouse bone marrow. Proc Natl Acad Sci USA 79:4665–4667

    Article  CAS  Google Scholar 

  • Schrader JW, Lewis SJ, Clark-Lewis I, Culvenor JG (1981) The persisting (P) cell: histamine content, regulation by a T cell-derived factor, origin from a bone marrow precursor, and relationship to mast cells. Proc Natl Acad Sci USA 78:323–327

    Article  CAS  Google Scholar 

  • Seeds MC, Bass DA (1999) Regulation and metabolism of arachidonic acid. Clin Rev Allergy Immunol 17:5–26

    Article  CAS  Google Scholar 

  • Shinomiya F, Hamauzu Y, Kawahara T (2009) Anti-allergic effect of a hot-water extract of quince (Cydonia oblonga). Biosci Biotechnol Biochem 73:1773–1778

    Article  CAS  Google Scholar 

  • Silva BM, Andrade PB, Valentão P, Ferreres F, Seabra RM, Ferreira MA (2004) Quince (Cydonia oblonga Miller) fruit (pulp, peel, and seed) and Jam: antioxidant activity. J Agric Food Chem 52:4705–4712

    Article  CAS  Google Scholar 

  • Siraganian RP, McGivney A, Barsumian EL, Crews FT, Hirata F, Axelrod J (1982) Variants of the rat basophilic leukemia cell line for the study of histamine release. Fed Proc 41:30–34

    CAS  Google Scholar 

  • Sostres C, Gargallo CJ, Arroyo MT, Lanas A (2010) Adverse effects of non-steroidal anti-inflammatory drugs (NSAIDs, aspirin and coxibs) on upper gastrointestinal tract. Best Pract Res Clin Gastroenterol 24:121–132

    Article  CAS  Google Scholar 

  • Stone KD, Prussin C, Metcalfe DD (2010) IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol 125:S73–S80

    Article  Google Scholar 

  • Williams CM, Galli SJ (2000) The diverse potential effecter and immunoregulatory roles of mast cells in allergic disease. J Allergy Clin Immunol 105:847–859

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was partly supported by a grant from the Shinshu Foundation for Promotion of Agricultural and Forest Science. I would like to express my sincere gratitude to Professor Hajime Otani, Laboratory of Food Bioscience, Faculty of Agriculture, Shinshu University, for providing me the opportunity to study in his laboratory. This work was also technically supported by the Collaborated Research Center for Food Functions, Faculty of Agriculture, Shinshu University (CREFAS) and the Research Center for Human and Environmental Sciences Division of Instrumental Analysis, Shinshu University.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Takeshi Kawahara.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s10616-011-9370-9

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kawahara, T., Iizuka, T. Inhibitory effect of hot-water extract of quince (Cydonia oblonga) on immunoglobulin E-dependent late-phase immune reactions of mast cells. Cytotechnology 63, 143–152 (2011). https://doi.org/10.1007/s10616-010-9323-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10616-010-9323-8

Keywords

  • Quince
  • Mast cell
  • Late-phase reaction
  • Leukotriene C4
  • Prostaglandin D2
  • Cyclooxygenase