, Volume 63, Issue 2, pp 119–131 | Cite as

Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus

  • Yupin Li
  • Takeki Hamasaki
  • Noboru Nakamichi
  • Taichi Kashiwagi
  • Takaaki Komatsu
  • Jun Ye
  • Kiichiro Teruya
  • Masumi Abe
  • Hanxu Yan
  • Tomoya Kinjo
  • Shigeru Kabayama
  • Munenori Kawamura
  • Sanetaka ShirahataEmail author
Original Research


Electrolyzed reduced water, which is capable of scavenging reactive oxygen species, is attracting recent attention because it has shown improved efficacy against several types of diseases including diabetes mellitus. Alloxan produces reactive oxygen species and causes type 1 diabetes mellitus in experimental animals by irreversible oxidative damage to insulin-producing β-cells. Here, we showed that electrolyzed reduced water prevented alloxan-induced DNA fragmentation and the production of cells in sub-G1 phase in HIT-T15 pancreatic β-cells. Blood glucose levels in alloxan-induced type 1 diabetes model mice were also significantly suppressed by feeding the mice with electrolyzed reduced water. These results suggest that electrolyzed reduced water can prevent apoptosis of pancreatic β-cells and the development of symptoms in type 1 diabetes model mice by alleviating the alloxan-derived generation of reactive oxygen species.


Electrolyzed reduced water Alloxan Type 1 diabetes mellitus Reactive oxygen species HIT-T15 cells 





Bovine serum albumin


Ethylenediaminetetraacetic acid


Electrolyzed reduced water


Fetal bovine serum


Diabetes mellitus


Hank’s balanced salt solution


4-[2-hydroxyethyl]-1-piperazineethane-sulfonic acid


Phosphate buffered saline


Propidium iodide


Reactive oxygen species


Type 1 diabetes mellitus


Type 2 diabetes mellitus


Terminal deoxynucleotidyl transferase



The authors thank Ms. Yuki Higuchi for excellent technical assistance.

Supplementary material

10616_2010_9317_MOESM1_ESM.doc (246 kb)
Supplementary material 1 (DOC 246 kb)


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Yupin Li
    • 1
    • 2
  • Takeki Hamasaki
    • 1
  • Noboru Nakamichi
    • 3
  • Taichi Kashiwagi
    • 1
  • Takaaki Komatsu
    • 1
  • Jun Ye
    • 1
    • 4
  • Kiichiro Teruya
    • 1
    • 5
  • Masumi Abe
    • 1
  • Hanxu Yan
    • 5
  • Tomoya Kinjo
    • 5
  • Shigeru Kabayama
    • 3
  • Munenori Kawamura
    • 6
  • Sanetaka Shirahata
    • 1
    • 5
    Email author
  1. 1.Department of Bioscience and Biotechnology, Faculty of AgricultureKyushu UniversityFukuokaJapan
  2. 2.School of Life SciencesNanchang University of Science and TechnologyNanchangPeople’s Republic of China
  3. 3.Nihon Trim Co LtdOsakaJapan
  4. 4.School of Life ScienceXiamen UniversityFujianPeople’s Republic of China
  5. 5.Graduate School of Systems Life SciencesKyushu UniversityFukuokaJapan
  6. 6.Kyowa HospitalKobeJapan

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