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Effects of hydroxyurea on monoclonal antibody production induced by anti-mIgG and LPS stimulation on murine B cell hybridomas

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Abstract

Chemical treatment with hydroxyurea (HU) has been selected as a simple and low cost strategy to generate a cell population enriched for the G1 phase. After the chemical treatment with HU, cells were stimulated with anti-mIgG to test if the positive effects of anti-mIgG on CD40 expression and specific IgG2a production rate were improved upon a cell population with a higher percentage of cells in G1 phase at the beginning of the cell culture. In addition, other treatments assayed in this work were the cell stimulation with Lipopolysaccharide (LPS) both before and after the HU treatment. It has been observed that the use of HU under conditions able to maintain the cells in viable state (0.1 mM for 20 h), has a negative effect on CD40 expression and specific IgG2a production rate induced by anti-mIgG. The positive effect of LPS on cell stimulation induced by anti-mIgG is reduced on cells treated with HU.

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Acknowledgments

We would like to thank the Spanish Ministry for Education and Science for conceding a Postgraduate Grant to A. Martín-López that has made this research possible. The authors acknowledge and appreciate the financial support received from Junta de Andalucía-Spain (P07-CVI-03193) and Ministerio de Ciencia e Innovación-Spain (BIO2008-06505).

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Almería, 04120, Almería, Spain

    Alicia Martín-López, Asterio Sánchez-Mirón, Francisco García-Camacho, Antonio Contreras-Gómez & Emilio Molina-Grima

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  1. Alicia Martín-López
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  2. Asterio Sánchez-Mirón
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  3. Francisco García-Camacho
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  4. Antonio Contreras-Gómez
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  5. Emilio Molina-Grima
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Correspondence to Antonio Contreras-Gómez.

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Martín-López, A., Sánchez-Mirón, A., García-Camacho, F. et al. Effects of hydroxyurea on monoclonal antibody production induced by anti-mIgG and LPS stimulation on murine B cell hybridomas. Cytotechnology 62, 205–215 (2010). https://doi.org/10.1007/s10616-010-9278-9

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