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Cytotoxic and genotoxic effects of sodium hypochlorite on human peripheral lymphocytes in vitro

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Abstract

Chlorination is widely used method in the disinfection of drinking and utility water worldwide. In this study, cytotoxic and genotoxic effects of sodium hypochlorite were investigated by the cytokinesis-block micronucleus assay and chromosomal aberration analysis on human peripheral lymphocytes in vitro. A significant increase in chromosomal aberration frequency was observed in all treatments of NaOCl (0.030, 0.065, 0.100, 0.25, 0.5, 1, 2, 4 μg/mL) at 24 and 48 h compared with the negative control and mitomycin C (MMC, 0.3 μg/mL), which was used as a positive control. NaOCl significantly increased the frequency of micronuclei in a dose dependent manner. The results showed that there was a significant correlation between NaOCl concentration and chromosomal aberration, micronuclei frequency, necrotic cells, apoptotic cells and binucleated cells.

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  1. Department of Biology, Faculty of Sciences and Arts, University of Kafkas, Kars, Turkey

    Süleyman Gül, Asu Savsar & Zeynep Tayfa

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  1. Süleyman Gül
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  2. Asu Savsar
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  3. Zeynep Tayfa
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Correspondence to Süleyman Gül.

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Gül, S., Savsar, A. & Tayfa, Z. Cytotoxic and genotoxic effects of sodium hypochlorite on human peripheral lymphocytes in vitro. Cytotechnology 59, 113–119 (2009). https://doi.org/10.1007/s10616-009-9201-4

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  • DOI: https://doi.org/10.1007/s10616-009-9201-4

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