, Volume 54, Issue 2, pp 71–76 | Cite as

Electroporation: an arsenal of application

  • Ti-Fei YuanEmail author


Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed, whose most important point is that it adopts low energy and rectangular pulse that could obtain high transfection efficiency and low damage to the embryo. This paper reviewed on the pool of application: from lab works to human clinical treatments.


Electroporation Gene transfer Electrotransfection Electrochemotherapy 



I am grateful to Dr. Ding, Y.Q. and Dr. Nakamura, H. for helpful comments on this manuscript and instructive discussions to my experiment.


  1. Araki I, Nakamura H (1999) Engrailed defines the position of dorsal di-mesencephalic boundary by repressing diencephalic fate. Development 126:5127–5135Google Scholar
  2. Akamatsu W, Okano HJ, Osumi N, Inoue T, Nakamura S, Sakakibara S, Miura M, Matsuo N, Darnell RB, Okano H (1999) Mammalian ELAV-like neuronal RNA-binding proteins Hub and Huc promote neuronal development in both the central and the peripheral nervous systems. Proc Natl Acad Sci USA 96:9885–9890CrossRefGoogle Scholar
  3. Arnold WM, Zimmermann U (1982) Rotating-field induced rotation and measurement of the membrane capacitance of single mesophyll cells of Avena sativa. Z Naturforsch 37c:908–915Google Scholar
  4. Baker PF, Knight DE (1978) Calcium-dependent exocytosis in bovine adrenal medullary cells with leaky plasma membrane. Nature 276:620–622CrossRefGoogle Scholar
  5. Baron S, Poast J, Rizzo D, McFarland E, Kieff E (2000) Electroporation of antibodies, DNA, and other macromolecules into cells: a high efficient method. J Immunol Med 242:115–126CrossRefGoogle Scholar
  6. Berglund DL, Starkey JR (1991) Introduction of antibody of into viable cells using electroporation. Cytometry 12:64–67CrossRefGoogle Scholar
  7. Briscoe J, Pierani A, Jessell TM, Ericson J (2000) A homeodomain protein code specifies progenitor cell identity and neuronal fate in the ventral neural tube. Cell 101:435–445CrossRefGoogle Scholar
  8. Buono RJ, Linser PJ (1992) Transient expression of RSVCAT in transgenic zebra fish made by electroporation. Mol Mar Biol Biotechnol 1:271–275Google Scholar
  9. Chakrabarti R, Wylie DE, Schuster SM (1989) Transfer of monoclonal antibodies into mammalian cells by electroporation. J Biol Chem 264:15494–15500Google Scholar
  10. Chang DC (1989a) Cell poration and cell fusion using an oscillating electric field. Biophys J 56:641–652Google Scholar
  11. Chang DC (1989b) Cell poration and cell fusion using and oscillating electric field. In: Neumann E (ed) Electroporation and electrofusion in cell biology. Plenum Publishing Corp., New York, pp 215–227Google Scholar
  12. Dagher SF, Conrad SE, Werner EA, Patterson RJ (1992) Phenotypic conversion of TK-deficient cells following electroporation of functional TK enzyme. Exp Cell Res 198:36–42CrossRefGoogle Scholar
  13. den Plas DV, Ponsaerts P, Tendeloo VV, Van Bockstaele DR, Berneman ZN, Merregaert J (2003) Efficient removal of Loxp-flanked genes by electroporation of Cre-recombinase mRNA. Biochem Biophy Res Commun 305:10–15CrossRefGoogle Scholar
  14. Dimitrov DS, Jain RK (1984) Membrane stability. Biochim Biophys Acta 779:437–468Google Scholar
  15. Dityateva G, Hammond M, Thiel C, Ruonala MO, Delling M, Siebenkotten G, Nix M, Dityatev A (2003) Rapid and efficient electroporation-based gene transfer into primary dissociated neurons. J Neurosci Meth 130:65–73CrossRefGoogle Scholar
  16. Eide FF, Eisenberg SR, Sanders TA (2000) Electroporation-mediated gene transfer in free-swimming embryonic Xenopus laevis. FEBS lett 486:29–32CrossRefGoogle Scholar
  17. Fromm ME, Taylor LP, Walbot V (1985) Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc Natl Acad Sci USA 82:5824–5828CrossRefGoogle Scholar
  18. Fromm ME, Taylor LP, Walbot V (1986) Stable transformation of maize after gene transfer by electroporation. Nature 319:1099–1103CrossRefGoogle Scholar
  19. Fox MB, Esveld DC, Valero A, Luttge R, Mastwijk HC, Bartels PV, van den Berg A, Boom RM (2006) Electroporation of cells in microfluidic devices: a review. Anal Bioanal Chem 560:1–23Google Scholar
  20. Funahashi J-I, Okafuji T, Ohuchi H, Noji S, Tanaka H, Nakamura H (1999) Role of Pax-5 in the regulation of a mid-hindbrain organizer’s activity. Dev Growth Differ 41:59–72CrossRefGoogle Scholar
  21. Ghartey-Tagoe EB, Babbin BA, Nusrat A, Neish AS, Prausnitz MR (2006) Plasmid DNA and siRNA transfection of intestinal epithelial monolayers by electroporation. Int J Pharm 315:122–133CrossRefGoogle Scholar
  22. Gould A, Itasaki N, Krumlauf R (1998) Initiation of rhombomeric Hoxb4 expression requires induction by somites and a retinoid pathway. Neuron 21:39–51CrossRefGoogle Scholar
  23. Hass K, Sin WC, Javaherian A, Li Z, Cline HT (2001) Single-cell electroporation for gene transfer in vivo. Neuron 29:583–591CrossRefGoogle Scholar
  24. Hashimoto H, Morikawa H, Yamada Y, Kimura A (1985) A novel method for transformation of intact yeast cells by electroinjection of plasmid DNA. AppMicrobiolBiotechnol 21:336–339Google Scholar
  25. Hashimoto K, Tatsumi N, Okuda K (1989) Introduction of phalloidin labeled with fluorescein isothiocyanate into living polymorphonuclear leukocytes by electroporation. J Biochem Biophys Methods 19:143–154CrossRefGoogle Scholar
  26. Hilgers V, Pourquié O, Dubrulle J (2005) In vivo analysis of mRNA stability using the Tet-Off system in the chicken embryo. Dev Biol 284:292–300CrossRefGoogle Scholar
  27. Huang Y, Rubinsky B (2000) Micro-electroporation: improving the efficiency and understanding of electrical permeabilisation of cells. Biomed Microdev 3:145–150Google Scholar
  28. Itasaki N, Bel-Vialar S, Krumlauf R (1999) ‘Shocking’ developments in chick embryology: electroporation and in vivo gene expression. Nat Cell Biol 1:E203–E207CrossRefGoogle Scholar
  29. Karube I, Tamiya E, Matsuoka J (1985) Transformation of Saccaromyces cerevisiae spheroplast by high electric pulse. FEBS lett 182:90–94CrossRefGoogle Scholar
  30. Kamdar PG, Von Allmen G, Finnerty V (1992) Transient expression of DNA in Drosophila via electroporation. Nucle Acid Res 20:3526CrossRefGoogle Scholar
  31. Katahira T, Sato T, Sugiyama S, Okafuji T, Araki I, Funahashi J, Nakamura H (2000) Interaction between Otx2 and Gbx2 defines the organizing center for the optic tectum. Mech Dev 91:43–52CrossRefGoogle Scholar
  32. Kinosita K Jr, Tsong TY (1977a) Hemolysis of human erythrocytes by transient electric field. Proc Natl Acad Sci USA 74:1923–1927CrossRefGoogle Scholar
  33. Kinosita, KJr, Tsong TY (1977b) Formation and resealing of pores of controlled size in human erythrocyte membrane. Nature(Lond) 268:438–441CrossRefGoogle Scholar
  34. Kinosita K Jr, Tsong TY (1977c) Voltage induced pore formation and hemolysis of human erythrocytes. Biochim Biophys Acta 471:227–242CrossRefGoogle Scholar
  35. Leclere PG, Panjwani A, Docherty R, Berry M, Pizzey J, Tonge DA (2005) Effective gene delivery to adult neurons by a modified form of electroporation. J Neurosci Meth 142:137–143CrossRefGoogle Scholar
  36. Leopold RA, Hughes KJ, Devault D (1996) Using electroporation and a slot cuvette to deliver plasmid DNA to insect embryos. Genet Anal/Biomol Eng 12:197–200CrossRefGoogle Scholar
  37. Lin YC, Huang MY, Li M (2002) Observation of extremely low transmembrane potential of cells in electroporation using microchips. MicroTAS’02, Nara, Japan, pp 847–849Google Scholar
  38. Lovell P, Jezzini SH, Moroz LL (2006) Electroporation of neurons and growth cones in Aplysia californica. J Neurosci Meth 151:114–120CrossRefGoogle Scholar
  39. Lukas J, Bartek J, Strauss M (1994) Efficient transfer of antibodies into mammalian cells by electroporation. J Immunol Med 170:255–259CrossRefGoogle Scholar
  40. Lundqvist JA, Sahlin F, Åberg MA, StrÖmberg A, Eriksson PS, Orwar O (1998) Altering the biochemical state of individual cultured cells and organelles with ultramicroelectrodes. Proc Natl Acad Sci USA 95:10356–10360CrossRefGoogle Scholar
  41. Luo JK, Redies C (2004) Overexpression of genes in Purkinje cells in the embryonic chicken cerebellum by in vivo electroporation. J Neurosci Meth 319:241–245CrossRefGoogle Scholar
  42. Luo JK, Redies C (2005) Ex ovo electroporation for gene transfer into older chicken embryos. Dev Dyn 233:1470–1477CrossRefGoogle Scholar
  43. Matsunaga E, Araki I, Nakamura H (2000) Pax6 defines the di-mesencephalic boundary by repressing En1 and Pax2. Development 127:2357–2365Google Scholar
  44. Mellitzer G, Hallonet M, Chen L, Ang SL (2002) Spatial and temporal ‘knock down’ of gene expression by electroporation of double-stranded RNA and morpholinos into early postimplantation mouse embryos. Mech Dev 118:57–63CrossRefGoogle Scholar
  45. Mir LM, Bureau MF, Gehl J, Rangara R, Rouy D, Caillaud JM, Delaere P, Branellec D, Schwartz B, Scherman D (1999) High efficiency gene transfer into skeletal muscle mediated by electric pulses. Proc Natl Acad Sci USA 96:4262–4267CrossRefGoogle Scholar
  46. Momose T, Tonegawa A, Takeuchi J, Ogawa H, Umesono K, Yasuda K (1999) Efficient targeting of gene expression in chick embryos by microelectroporation. Dev Growth Differ 41:335–344CrossRefGoogle Scholar
  47. Mori K, Hasegawa T, Sato T, Sugibayashi K (2003) Effect of electric field on the enhanced skin permeation of drugs by electroporation. J Control Release 90:171–179CrossRefGoogle Scholar
  48. Moto K, Abdel Salam SE, Sakurai S, Iwami M (1999) Gene transfer into insect brain and cell-specific expression of Bombyxin gene. Dev Genes Evol 209:447–450CrossRefGoogle Scholar
  49. Muller F, Lele Z, Varadi L, Menczel L, Orban L (1993) Efficient transient expression system based on square pulse electroporation and in vivo luciferase assay of fertilized fish eggs. FEBS Lett 324:27–32CrossRefGoogle Scholar
  50. Muramatsu T, Mizutani Y, Okumura J (1996) Live detection of the firefly luciferase gene expression in early chicken embryos by bioluminescence in incubating chicken embryos. Ann Sci Technol 67:906–909Google Scholar
  51. Neumann E, Schaefer-Riddder M, Wang Y, Hofschneider PH (1982) Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO (Eur Mol biol Organ) J.1:841–845Google Scholar
  52. Neumann E, Kakorin S, Toensing K (2000a) In: Jaroszeski MJ, Heller R, Gilbert R (eds) Electrochemotherapy, electrogenetherapy and transdermal drug delivery. The Human Press Inc., Clifton, UK, pp 1–35Google Scholar
  53. Neumann E, Tonsing K, Siemens P, (2000b). Perspectives for microelectrode arrays for biosening and membrane electroporation. Bioelectrochemistry 51:125–132CrossRefGoogle Scholar
  54. Nolkrantz K, Farre C, Brederlau A, Karlsson RI, Brennan C, Eriksson PS, Weber SG, Sandberg M, Orwar O (2001) Electroporation of single cells and tissues with an electrolyte-filled capillary. Anal Chem 73:4469–4477CrossRefGoogle Scholar
  55. Olofsson J, Nolkrantz K, Ryttsén F, Lambie BA, Weber SG, Orwar O (2003) Single-cell electroporation. Curr Opin Biotech 14:29–34CrossRefGoogle Scholar
  56. Osumi N, Inoue T (2001) Gene transfer into cultured mammalian embryos by electroporation. Methods 24:35–42CrossRefGoogle Scholar
  57. Ou-Lee TM, Turgeon R, Wu R (1986) Expression of a foreign gene linked to either a plant -virus or a Drosophila promoter after electroporation of protoplasts of rice, wheat, and sorghum. Proc Natl Acad Sci USA 83:6815–6819CrossRefGoogle Scholar
  58. Prechtel AT, Turza NM, Theodoridis AA, Kummer M, Steinkasserer A (2006) Small interfering RNA (siRNA) delivery into monocyte-derived dendritic cells by electroporation. J Immuno Meth 311:139–152CrossRefGoogle Scholar
  59. Rols MP, Delteil C, Golzio M, Dumond P, Cros S, Teissie J (1998) In vivo electrically mediated protein and gene transfer in murine melanoma. Nat Biotechnol 16:168–171CrossRefGoogle Scholar
  60. Rui M, Chen Y, Zhang Y, Ma D (2002) Transfer of anti-TFAR19 monoclonal antibody into HeLa cells by in situ electroporation can inhibit the apoptosis. Life sciences 71:1771–1778CrossRefGoogle Scholar
  61. Saito T, Nakatsuji N (2001) Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Dev Biol 240:237–246CrossRefGoogle Scholar
  62. Schwan HP (1989) Dielectrophoresis and rotation of cells. In: Electroporation and electrofusion in cell biology. Plenum Press, New York, pp 3–21Google Scholar
  63. Shin YS, Cho K, Kim JK, Lim SH, Park CH, Lee KB, Park Y, Chung C, Han DC, Chang JK (2004) Electrotransfection of mammalian cells using microchannel-type electroporation chip. Anal Chem 76:7045–7052CrossRefGoogle Scholar
  64. Shivarova N, Foster W, Jacob HE, Grigorova R (1983) Microbiological implications of electric field effects. Z Alleg Microbiol 23:595–599CrossRefGoogle Scholar
  65. Sowers AE (1984) Characterization of electric field induced fusion in erythrocyte ghost membranes. J Cell Biol 99:1989–1996CrossRefGoogle Scholar
  66. Sugar IP, Forster W, Neumann E (1987) Model of cell electrofusion, membrane pores. Biophys Chem 19:211–225CrossRefGoogle Scholar
  67. Swartz M, Eberhart J, Mastick GS, Krull CE (2001) Sparking New Frontiers: Using in vivo electroporation for genetic manipulations. Dev Biol 233:13–21CrossRefGoogle Scholar
  68. Tabata H, Nakajima K (2001) Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex. Neuroscience 103:865–872CrossRefGoogle Scholar
  69. Thomas JL (2003) Electroporation, an alternative to biolistics for transfection of Bombyx mori embryos and larval tissues. J Insect Sci 3:1–12CrossRefGoogle Scholar
  70. Tjelle TE, Salte R, Mathiesen I, Kjeken R. A novel electroporation device for gene delivery in large animal and humans. Vaccine (in press)Google Scholar
  71. Traas JA, Doonan JH, Rawins DJ, Shaw PJ, Watts J, Lloyd CW (1987) An actin network is present in the cytoplasm throughout the cell cycle of carrot cells and associated with the dividing nucleus. J Cell Biol 105:387–395CrossRefGoogle Scholar
  72. Uchikawa M, Takemoto T, Kamachi Y, Kondoh H (2004) Efficient identification of regulatory sequences in the chicken genome by a powerful combination of embryo electroporation and genome comparison. Mech Dev 121:1145–1158CrossRefGoogle Scholar
  73. Vicat JM, Boisseau S, Jourdes P, Laine M, Wion D, Bouali-Banazzouz R, Benabid AL, Berger F (2000) Muscle transfection by electroporation with high-voltage and short-pulse currents provides high-level and long-lasting gene expression. Hum Gene Ther 11:909–916CrossRefGoogle Scholar
  74. Wang HM, Xie TD (2000) The theoretical technology and application of cell electroporation, electrofusion and electrostimulation. TianJinKeXueJiShu Press, Tianjin, China, 23Google Scholar
  75. Watanabe Y, Nakamura H (2000) Control of chick tectum territory along dorsoventral axis by Sonic hedgehog. Development 127:1131–1140Google Scholar
  76. Wei F, Xia XM, Tang J, Ao H, Ko S, Liauw J, Qiu CS, Zhuo M (2003) Calmodulin regulates synaptic plasticity in the anterior cingulate cortex and behavioral responses: a microelectroporation study in adult rodents. J Neurosci 23:8402–8409Google Scholar
  77. Whelan J (2002) Electroporation and ultrasound for gene and drug delivery. Drug Discov Today 7:585–586CrossRefGoogle Scholar
  78. Winegar RA, Philips JW, Youngblom JH et al (1989) Cell electroporation is a highly efficient method for introducing restriction endonucleases into cells. Mutat Res 225:49–53CrossRefGoogle Scholar
  79. Wong TK, Neumann E (1982) Electric field mediated gene transfer. Biochem Biophys Res Commun 107:584–587CrossRefGoogle Scholar
  80. Xiang L, Murai A, Muramatsu T (2004) The effects of agouti-related protein gene transfer in vivo by electroporation in mice. Neurosci Lett 370:108–113CrossRefGoogle Scholar
  81. Yorifuji T, Mikawa H (1990) Co-transfer of restriction endonucleases and plasmid DNA into mammalian cells by electroporation: Effects on stable transformation. Mutat Res 243:121–126 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Department of Biological Science and Biotechnology, Life Science SchoolSun Yat-Sen (ZhongShan) UniversityGuangzhouChina

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