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Cytotechnology

, 50:109 | Cite as

Current status of transcriptional regulation systems

  • Tobias May
  • Hansjörg HauserEmail author
  • Dagmar Wirth
original Paper

Abstract

Many attempts have been undertaken to control transgene activity in mammalian cells. This is of importance for both applied biotechnology and basic research activities. State of the art regulatory systems use elements for transgene regulation which are unrelated to host regulatory networks and thus do not interfere with endogenous activities. Most of these regulation systems consist of transregulators and transregulator responding promoter elements that are derived from non mammalian origin. Apart from the tetracycline (Tet) regulated system which is most widely used for conditional gene expression at the moment, a number of new systems were created. These systems have been significantly refined and their performance makes them suitable for regulating transgenes not only in cellular systems but also in transgenic animals and for human therapeutic use.

Keywords

Viral transduction Autoregulatory expression Test-system Synthetic promotor Gene regulation 

Abbreviations

eGFP

 Enhanced green fluorescent protein

EPO

 Recombinant human erythropoietin

GOI

 Gene of interest

IRES

 Internal ribosomal entry site

lmw

 Low molecular weight

pA

 Polyadenylation site

PTA

 Transactivator dependent promoter

SEAP

 Secreted form of human placental alkaline phosphatase

TAg

 Simian virus 40 large T antigen

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Department of Gene Regulation and DifferentiationGBF—German Research Center for BiotechnologyBraunschweigGermany

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