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Cytotechnology

, 50:49 | Cite as

The role of recombinant proteins in the development of serum-free media

  • Joanne KeenanEmail author
  • Dermot Pearson
  • Martin Clynes
Review

Abstract

Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly animal-derived. Given recent concerns about TSEs (transmissible spongiform encephalopathies) and other adventitious agents, the drive in media formulations must be towards elimination of animal-origin materials while maintaining cell line productivity. The progress made towards removing animal-derived components and the use of recombinant proteins in serum-free media for mammalian cells is reviewed.

Keywords

Transferrin Bovine Spongiform Encephalopathy Madin Darby Canine Kidney Madin Darby Canine Kidney Cell Adventitious Agent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.National Institute for Cellular BiotechnologyDublin City UniversityDublinIreland
  2. 2.Delta Biotechnology Ltd.NottinghamUK

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