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Cytotechnology

, Volume 49, Issue 2–3, pp 143–151 | Cite as

Transcriptional Regulation of the α-fetoprotein Gene by SWI/SNF Chromatin Remodeling Complex

  • Katsuhide Miyake
  • Mikio Takahashi
  • Takeaki Dohda
  • Hidenori Kaneoka
  • Yoshitaka Sato
  • Yujin Inayoshi
  • Masamichi Kamihira
  • Shinji IijimaEmail author
Article
  • 47 Downloads

Abstract

α-Fetoprotein (AFP) is a fetal serum protein abundant in fetal liver whose expression is downregulated in adult liver. The promoter–proximal region of the AFP gene contains two binding sites for CCAAT/enhancer-binding protein α (C/EBPα), two for hepatocyte nuclear factor-1α (HNF-1α) and one for nuclear factor-1 (NF-1). Luciferase reporter assays showed that a combination of C/EBPα, HNF-1α, NF-1 and coactivator p300 gave the maximal activity but the BRG1 and BRM, ATPase subunit of the chromatin remodeling complex SWI/SNF, repressed the transactivation of the AFP gene by these transcription factors. Deletion analyses of C/EBPα binding sites suggested that C/EBPα recruited SWI/SNF complex and caused the repression. This repression may also play important roles in the downregulation of the AFP gene in adult hepatocytes.

Keywords

α-Fetoprotein CCAAT enhancer binding protein Chromatin remodeling complex Transcriptional control 

Abbreviations

AFP

α-Fetoprotein

C/EBPα

CCAAT enhancer binding protein α

COUP-TF

chicken ovalbumin upstream promoter–transcription factor

HNF-1α

hepatocyte nuclear factor-1α

HNF3

hepatocyte nuclear factor-3

NF-1

nuclear factor-1

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Copyright information

© Springer 2006

Authors and Affiliations

  • Katsuhide Miyake
    • 1
    • 2
  • Mikio Takahashi
    • 1
  • Takeaki Dohda
    • 1
  • Hidenori Kaneoka
    • 1
  • Yoshitaka Sato
    • 1
  • Yujin Inayoshi
    • 1
  • Masamichi Kamihira
    • 1
  • Shinji Iijima
    • 1
    Email author
  1. 1.Department of Biotechnology, Graduate School of EngineeringNagoya UniversityNagoyaJapan
  2. 2.Ecotopia Science InstituteNagoya UniversityNagoyaJapan

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