An Optimized Protocol for Culture of Cardiomyocyte from Neonatal Rat
- 1.2k Downloads
Primary culture of cardiomyocytes has been widely used as a valuable tool for pharmacological and toxicological studies. However, the fact that heart is a solid organ and cardiomyocytes do not proliferate after birth makes the primary myocardial culture a tedious job. The present study reports an improved method for rapid isolation of cardiomyocytes, as well as the culture maintenance and quality assurance. The whole culture process can be shortened to 3.5 h by reducing enzyme digestion period. Moreover, the new protocol guarantees cell yield and viability, and produces more than 95% cardiomyocytes in culture. The cardiomyocytes can respond to Angiotension II stimulation with increased protein synthesis, suggesting the practical value of this new culture method.
KeywordsCardiomyocytes Culture Neonatal Precondition Protocol
Unable to display preview. Download preview PDF.
- Chlopclkova, S., Psotova, J., Miketova, P. 2001Neonatal rat cardiomyocytes – a model for the study of morphological, biochemical and electrophysiological characteristic of the heartBiomed. Papers1454955Google Scholar
- Harry, L., Farley, B. 1960In vitro studies of single isolated beating heart cellsScience13116741675Google Scholar
- Landes, M., Gaumann, A., Laue, C., Schrezenmeir, J. 2001Improved yield and functionality of parathyroid cells separated by using collagenase-digestion with cold pre- incubationJ. Endocrinol. Invest.2498103Google Scholar
- Laue, C., Hakimi, A., Schrenmetr, J. 1995Islet Single-cells Preparation using Cold Preincubation With Trypsin, Fifth International Congress on Pancreas and Islet TransplantationMiami BeachFloridaUSAGoogle Scholar
- Shields, P.P., Dixon, J.E., Glembotski, C.C. 1988The secretion of atrial natriuretic factor-(99–126) by cultured cardiac myocytes is regulated by glucocorticoidsJ. Biol. Chem.2631261928Google Scholar