Advertisement

Cytotechnology

, Volume 49, Issue 2–3, pp 109–116 | Cite as

An Optimized Protocol for Culture of Cardiomyocyte from Neonatal Rat

  • Jiajia Fu
  • Jie Gao
  • Rongbiao Pi
  • Peiqing LiuEmail author
Article

Abstract

Primary culture of cardiomyocytes has been widely used as a valuable tool for pharmacological and toxicological studies. However, the fact that heart is a solid organ and cardiomyocytes do not proliferate after birth makes the primary myocardial culture a tedious job. The present study reports an improved method for rapid isolation of cardiomyocytes, as well as the culture maintenance and quality assurance. The whole culture process can be shortened to 3.5 h by reducing enzyme digestion period. Moreover, the new protocol guarantees cell yield and viability, and produces more than 95% cardiomyocytes in culture. The cardiomyocytes can respond to Angiotension II stimulation with increased protein synthesis, suggesting the practical value of this new culture method.

Keywords

Cardiomyocytes Culture Neonatal Precondition Protocol 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Blondel, B., Roijen, I., Cheneval, J.P. 1971Heart cells in culture: a simple method for increasing the proportion of myoblastsExperientia27356358PubMedCrossRefGoogle Scholar
  2. Chlopclkova, S., Psotova, J., Miketova, P. 2001Neonatal rat cardiomyocytes – a model for the study of morphological, biochemical and electrophysiological characteristic of the heartBiomed. Papers1454955Google Scholar
  3. Flanders, K.C., Holder, M.G., Winokur, T.S. 1995Autoinduction of mRNA and protein expression for ransforming growth factor-beta S in cultured cardiac cellsJ. Mol. Cell Cardiol.27805812PubMedCrossRefGoogle Scholar
  4. Gorelik, J., Shevchuk, A., Swiet, M.,  et al. 2004Comparison of the arrhythmogenic effects of tauro- and glycoconjugates of cholic acid in an in vitro study of rat cardiomyocytesBJOG111867870PubMedGoogle Scholar
  5. Harada, M., Saito, Y., Kuwahara, K.,  et al. 1998Interacion of myocytes and nonmyocytes is necessary for mechanical stretch to induce ANP/BNP production in cardiocyte cultureJ. Cardiovasc. Pharmacol. [suppl1]31S357S359CrossRefGoogle Scholar
  6. Harary, I., Farley, B. 1963In vitro studies on single beating rat heart cells II Intercellular communicationExp. Cell Res.29466474PubMedCrossRefGoogle Scholar
  7. Harry, L., Farley, B. 1960In vitro studies of single isolated beating heart cellsScience13116741675Google Scholar
  8. Landes, M., Gaumann, A., Laue, C., Schrezenmeir, J. 2001Improved yield and functionality of parathyroid cells separated by using collagenase-digestion with cold pre- incubationJ. Endocrinol. Invest.2498103Google Scholar
  9. Laue, C., Hakimi, A., Schrenmetr, J. 1995Islet Single-cells Preparation using Cold Preincubation With Trypsin, Fifth International Congress on Pancreas and Islet TransplantationMiami BeachFloridaUSAGoogle Scholar
  10. Mark, G.E., Strasser, F.F. 1966Pacemaker activity and mitosis in cultures of newborn rat heart ventricle cellsExp. Cell Res.44217233PubMedCrossRefGoogle Scholar
  11. Orlowski, J., Lingrel, J. 1990Thyroid and glucocorticoid hormones regulate the expression of multiple NaK-ATPase genes in cultured neonatal rat cardiac myocytesJ. Biol. Chem.26534623470PubMedGoogle Scholar
  12. Shields, P.P., Dixon, J.E., Glembotski, C.C. 1988The secretion of atrial natriuretic factor-(99–126) by cultured cardiac myocytes is regulated by glucocorticoidsJ. Biol. Chem.2631261928Google Scholar
  13. Simpson, P. 1985of hypertrophy of cultured neonatal rat heart cells through an α1-adrenergic receptor and induction of beating through an α1- and β1-adrenengic receptor interactonCirculation Res.5688494PubMedGoogle Scholar
  14. Simpson, P., Savion, S. 1982Differentiation of rat myocytes in single cell cultures with and without proliferating nonmyocardial cells. Cross-striations, ultrastructureand chronotropic response to isoproterenolCirc. Res.50101116PubMedGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Department of Pharmacology and ToxicologySun Yat-sen UniversityGuangzhouChina

Personalised recommendations