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Cytotechnology

, 50:141 | Cite as

Cell Culture Processes for the Production of Viral Vectors for Gene Therapy Purposes

  • James N. Warnock
  • Otto-Wilhelm Merten
  • Mohamed Al-RubeaiEmail author
Article

Abstract

Gene therapy is a promising technology for the treatment of several acquired and inherited diseases. However, for gene therapy to be a commercial and clinical success, scalable cell culture processes must be in place to produce the required amount of viral vectors to meet market demand. Each type of vector has its own distinct characteristics and consequently its own challenges for production. This article reviews the current technology that has been developed for the efficient, large-scale manufacture of retrovirus, lentivirus, adenovirus, adeno-associated virus and herpes simplex virus vectors.

Key words

Adeno-associated virus Adenovirus Bioreactor Culture conditions Herpes simplex virus vectors Lentivirus Microcarrier Optimization Process development Retrovirus 

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Copyright information

© Springer 2006

Authors and Affiliations

  • James N. Warnock
    • 1
  • Otto-Wilhelm Merten
    • 2
  • Mohamed Al-Rubeai
    • 3
    Email author
  1. 1.Department of Agricultural and Biological EngineeringMississippi State UniversityMississippi StateUSA
  2. 2.GénéthonEvry-CedexFrance
  3. 3.School of Chemical and Bioprocess EngineeringUniversity College DublinBelfieldIreland

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