, Volume 47, Issue 1–3, pp 51–57 | Cite as

Preparation of Cationic Immunovesicles Containing Cationic Peptide Lipid for Specific Drug Delivery to Target Cells

  • Takuya Sugahara
  • Shinichi Kawashima
  • Akiko Oda
  • Yoshio Hisaeda
  • Keiichi KatoEmail author


The cationic vesicle composed of Span80 and cationic peptide lipid (CPL) was prepared. The cytotoxicity of the Span80-CPL cationic vesicle was very low compared with Span80 vesicle. Antibody was able to be immobilized on vesicle surface by mediation of protein A. The antigen targeting ability of the antibody-immobilized vesicle (immunovesicle) derived from antibody was evaluated. Our results suggested that the Span80-CPL immunovesicles specifically associate with target cells by the antibody mediation, and the substance capsulated in immunovesicle was transferred into the target cells. This means that the Span80-CPL immunovesicle is expected to achieve a high local concentration of an encapsulated drug at the target.


Lipid Peptide Drug Delivery Target Cell Local Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



cationic peptide lipid


fetal bovine serum


fluorescein isothiocyanate


isothiocyanic acid dodecyl ester


phosphate buffered saline


propidium iodide


sorbitan monooleate


polyoxyethylene (20) sorbitan monooleate


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Copyright information

© Springer 2005

Authors and Affiliations

  • Takuya Sugahara
    • 1
  • Shinichi Kawashima
    • 2
  • Akiko Oda
    • 1
  • Yoshio Hisaeda
    • 3
  • Keiichi Kato
    • 2
    Email author
  1. 1.Faculty of AgricultureEhime UniversityMatsuyamaJapan
  2. 2.Faculty of EngineeringEhime UniversityMatsuyamaJapan
  3. 3.Faculty of EngineeringKyushu UniversityFukuokaJapan

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