Advertisement

Cytotechnology

, 49:87 | Cite as

The Effect of α-pinene from Pinus densiflora S. and a Polysaccharide from Angelica gigas Nakai on Differentiation and Proliferation of Human Embryonic Stem Cells

  • Seo H. Lee
  • Hyun Soo Lee
  • Hyung Cheol Moon
  • Dae Ho Kim
  • Young Shik Park
  • Baek Hwang
  • Hyeon Y. LeeEmail author
Article

Abstract

The leukemia inhibitory factor (LIF), which is a very expensive reagent, can be used to efficiently control the differentiation of human embryonic stem (ES) cells at concentrations >1000 units/ml for 6–7 days. However, in supplement <500 units/ml, most ES cells differentiate within 3–4 days in in vitro cultures. α-Pinene from Pinus densiflora S. and a polysaccharide (MW 25 kDa) from A. gigas Nakai showed promising results as a substitute for LIF in cultivating ES cells. By adding both 0.5 (μg/ml) of α-pinene and the polysaccharide, most of the ES cells could be maintained under undifferentiated conditions after adding only 100 units/ml of LIF. It was found that α-pinene can play a role in preventing the ES cells from differentiating and the polysaccharide can be used to grow the ES cells. The results suggest that human ES cells can be maintained under undifferentiated conditions by supplementing both plant extracts, which can result in a reduction in the amount of LIF needed.

Keywords

α-Pinene Angelica gigas Nakai Differentiation Human embryonic stem cell Pinus densiflora S. Polysaccharide Telomerase activity 

References

  1. Ahn, K.S., Sim, W.S., Kim, I.H. 1996Decursin a cytotoxic agent and protein kinase C activator from the root of Angelica gigasPlanta Med.6279PubMedGoogle Scholar
  2. Ahn, K.S., Sim, W.S., Kim, H.M., Han, S.B., Kim, H.H. 1998Immuno- stimulating polysaccharide from cell culture of A. gigas NakaiBiotech Lett.2057CrossRefGoogle Scholar
  3. Allsopp, R.D., Vaziri, H., Patterson, C., Goldstein, S., Younglai, E.V., Futcher, A.B., Harley, C.B. 1992Telomere length predicts replicative capacity of human fibroblastsProc. Natl. Acad. USA891011410118CrossRefGoogle Scholar
  4. Altman, P. 1989Australian tea tree oilAust. J. Biotechnol3217248Google Scholar
  5. Andrews, P.W. 1981Pluripotent cell line from early mouse embryos cultured in medium conditioned by tetracarcinoma stem cellsProc. Natl. Acad. Sci. USA7876347638CrossRefGoogle Scholar
  6. Becker, R.O. 2002Induced dedifferentiation: a possible alternative to embryonic stem cell transplantsNeuroRehabilitation172331PubMedGoogle Scholar
  7. Belldegrun, A., Kasid, A., Uppenkamp, M., Topalian, S., Rosenberg, S. 2001 In vitro cultivation of human ES cellsJ. Immunol14245204526Google Scholar
  8. Brower, V. 2001Technologies for regenerative medicineGenet. Eng. News21812Google Scholar
  9. Evans, M.J., Kaufman, M. 1981Establishment in culture of pluripotential stem cells from mouse embryosNature292151156Google Scholar
  10. Fong, C.Y., Bongso, A. 1999Comparison of human blastulation rates and total cell number in sequential culture media with and without co-cultureHum. Reprod.14774781PubMedCrossRefGoogle Scholar
  11. Gluckman, E., Bullock, J.K. 1997Outcomes of cord-blood transplantation from related and unrelated donorsN. Eng. J. Med.337373379CrossRefGoogle Scholar
  12. Ham, M.S., Kim, S.S., Hong, J.S., Lee, J.H., Chung, E.K., Lee, H.Y. 1996Screening and comparison of active substances of A. gigas Nakai produced in Kangwon and A. acutiloba produced in JapanKor. J. Appl. Microbiol. Biotechnol.24624629Google Scholar
  13. Harely, C.B., Futcher, A.B., Greider, C.W. 1990Telomere shorten during ageing of human fibroblastsNature345458460CrossRefGoogle Scholar
  14. Harley, C.B., Villeponteau, B. 1995Telomeres and telomerase in aging and cancerCurr. Opin. Genet. Develop.5249255CrossRefGoogle Scholar
  15. Hogan, B., Beddington, R., Constantini, E., Lacy, E. 1994Isolation, culture and manipulation of embryonic stem cellsLaboratory Manual. 2nd ed.Cold Spring Harbor Laboratory PressPlainview, NY254290Google Scholar
  16. Hong, J.S., Lee, J.N., Kim, S.H., Park, K.Y., Cho, J.S., Lee, H.Y. 1999The changes of growth patterns and production of BDNF in perfusioin cultivation of human neuroblastoma cellsJ. Microbiol. Biotechnol.9323327Google Scholar
  17. Kallos, M.S., Reynolds, B.A., Vescovi, A.L., Behie, L.A. 1998High Cell Density Growth of Mammalian Neural Stem Cells as Aggregates in BioreactorsFluidization IX, Public Engineering FoundationNew York145Google Scholar
  18. Kim, K.Y., Davidson, P.M., Chung, H.J. 2000Antimicrobial effectiveness of pine needles on foodborne illness bacteriaJ. Microbiol. Biotechnol.10227232CrossRefGoogle Scholar
  19. Kim, M.H., Chung, W.T., Kim, Y.K., Lee, J.H., Lee, H.Y. 2001The effect of the oil of A. rugosa Kuntze and three its components on human cancer cell linesJ. Essent. Oil Res.13214218Google Scholar
  20. Kim, N.W., Piatyszek, M.A., Prowse, K.R., Harley, C.B., West, M.D., Ho, P.L.C., Coviello, G.M., Wright, W.E., Shay, J.W. 1994Specific association of human telomerase activity with immortal cells and cancerScience26620112015PubMedGoogle Scholar
  21. Kumazawa, Y., Mizunoe, K., Otsuka, Y. 1982Immunostimulating polysaccharide separated from hot water extract of A. acutiloba.Immunology477583PubMedGoogle Scholar
  22. Kurtzberg, J. 1996Placental blood as a source of hematopoitic stem cells for transplantation into unrelated recipientsNew. Engl. J. Med.335157162PubMedCrossRefGoogle Scholar
  23. Li, J.H., Wei, Y., Wagner, T.E. 1999 In vitro endothelial differentiation of long-term cultured murine embryonic cells induced by matrigelStem Cells177281PubMedCrossRefGoogle Scholar
  24. Ling, V., Neben, S. 1997In vitro differentiation of embryonic stem cells: Immunophenotypic analysis of cultured embryoid bodiesJ. Cell Physiol.171104115PubMedCrossRefGoogle Scholar
  25. Marshall, E. 1998A versatile cell line raises scientific hopes, legal questionsScience28210141015PubMedCrossRefGoogle Scholar
  26. Reubinoff, B.E., Pera, M.F., Fong, C.Y., Trounson, A., Bongso, A. 2000Embryonic stem cell lines from human blastocysts: somatic differentiation in vitroNature/Biotech.18399404Google Scholar
  27. Rose, T.M., Weiford, D.M., Gunderson, N.L., Bruce, A.G. 1994Oncostatin M inhibits the differentiation of pluropotent embryonic stem cells in vitroCytokine64854PubMedCrossRefGoogle Scholar
  28. Schuldiner, M., Yanuka, O., Itskovitz, J.E., Melton, D.A., Benvenisty, N. 2000Effect of eight growth factors on the differentiation of cells derived from human embryonic stem cellsProc. Natl. Acad. Sci. USA971130711312PubMedCrossRefGoogle Scholar
  29. Shamblott, M.J., Axelman, J., Littlefield, J.W., Blumenthal, P.D., Huggins, G.R., Cui, Y.,, Cheng, L., Gearhart, J.D. 2001Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and porliferate extensively in vitroProc. Natl. Acad. Sci. USA98113118PubMedCrossRefGoogle Scholar
  30. Thomson, J.A., Itskovitz, E.J., Shapiro, S.S., Watknitz, M.A., Swiergiel, J.J., Jones, J.M. 1998Embryonic stem cell lines derived from human blastocystsScience28211451147PubMedCrossRefGoogle Scholar
  31. Thomson, J.A., Kalishman, J., Golos, T.G., Durning, M., Harris, C.P., Hearn, J.P. 1995Isolation of a primate embryonic stem cell lineProc. Natl. Acad. Sci. USA9278447848PubMedCrossRefGoogle Scholar
  32. Wang, N.L., Kiyohara, H., Matsumoto, T., Ostuka, H., Yamada, H. 1994Poloyclonal antibody against a complement-activating pectin from the roots of A. acutilobaPlanta Med.60425429PubMedGoogle Scholar
  33. Whish, J.P. 1996A flexible distillation system for the isolation of essential oilsJ. Essent. Oil Res.8405410Google Scholar
  34. Zhiming, K., Zhengtao, L., Bangyu, D. 1995Study on the antimutagenic effect of pine needle extractMutat. Res.347103109Google Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Seo H. Lee
    • 1
  • Hyun Soo Lee
    • 1
  • Hyung Cheol Moon
    • 1
  • Dae Ho Kim
    • 1
  • Young Shik Park
    • 1
  • Baek Hwang
    • 2
  • Hyeon Y. Lee
    • 1
    Email author
  1. 1.School of Biotechnology and BioengineeringKangwon National UniversityChunchonKorea
  2. 2.Department of BiologyChonnam National UniversityGwangjuKorea

Personalised recommendations