, 49:87 | Cite as

The Effect of α-pinene from Pinus densiflora S. and a Polysaccharide from Angelica gigas Nakai on Differentiation and Proliferation of Human Embryonic Stem Cells

  • Seo H. Lee
  • Hyun Soo Lee
  • Hyung Cheol Moon
  • Dae Ho Kim
  • Young Shik Park
  • Baek Hwang
  • Hyeon Y. LeeEmail author


The leukemia inhibitory factor (LIF), which is a very expensive reagent, can be used to efficiently control the differentiation of human embryonic stem (ES) cells at concentrations >1000 units/ml for 6–7 days. However, in supplement <500 units/ml, most ES cells differentiate within 3–4 days in in vitro cultures. α-Pinene from Pinus densiflora S. and a polysaccharide (MW 25 kDa) from A. gigas Nakai showed promising results as a substitute for LIF in cultivating ES cells. By adding both 0.5 (μg/ml) of α-pinene and the polysaccharide, most of the ES cells could be maintained under undifferentiated conditions after adding only 100 units/ml of LIF. It was found that α-pinene can play a role in preventing the ES cells from differentiating and the polysaccharide can be used to grow the ES cells. The results suggest that human ES cells can be maintained under undifferentiated conditions by supplementing both plant extracts, which can result in a reduction in the amount of LIF needed.


α-Pinene Angelica gigas Nakai Differentiation Human embryonic stem cell Pinus densiflora S. Polysaccharide Telomerase activity 


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Copyright information

© Springer 2006

Authors and Affiliations

  • Seo H. Lee
    • 1
  • Hyun Soo Lee
    • 1
  • Hyung Cheol Moon
    • 1
  • Dae Ho Kim
    • 1
  • Young Shik Park
    • 1
  • Baek Hwang
    • 2
  • Hyeon Y. Lee
    • 1
    Email author
  1. 1.School of Biotechnology and BioengineeringKangwon National UniversityChunchonKorea
  2. 2.Department of BiologyChonnam National UniversityGwangjuKorea

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