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Antimicrobial Metabolites from Extremophilic Fungus Botryotrichum piluliferum Strain WESH19

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Chemistry of Natural Compounds Aims and scope

Chromatographic analysis of a crude ethyl acetate (EtOAc) extract derived from a solid rice culture incubated at 40°C to mimic the natural ecological niche of the extremophilic fungus Botryotrichum piluliferum strain WESH19 yielded a new natural butenolide compound (1) together with three known metabolites (2–4). Chemical structures of the isolated metabolites were confirmed by HR-ESI-MS along with 1D and 2D NMR spectroscopic analyses. Compounds 1–3 revealed pronounced antimicrobial activities against Staphylococcus aureus (ATCC 700699), Enterococcus faecalis (ATCC 29212), and Enterococcus faecium (ATCC 35667), while their antifungal activities were assessed against the extremophilic fungus Penicillium simplicissimum strain WSH17 (GenBank Acc. No. MN055685). This study adds another example of how extreme environmental conditions may influence secondary metabolism of the inhabiting fungi.

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References

  1. G. F. Bills and J. B. Gloer, Biologically Active Secondary Metabolites from the Fungi, in: The Fungal Kingdom, J. Heitman, B. J. Howlett, P. W. Crous, E. H. Stukenbrock, T. Y. James, and N. A. R. Gow (eds.), American Society of Microbiology, Sterling, USA, 2017, pp. 1087–1119.

  2. R. Orellana, C. Macaya, G. Bravo, F. Dorochesi, A. Cumsille, R. Valencia, C. Rojas, and M. Seeger, Front. Microbiol., 9, 2309 (2018).

    Article  Google Scholar 

  3. R. Maheshwari, G. Bharadwaj, and M. K. Bhat, Microbiol. Mol. Biol. Rev., 64, 461 (2000).

    Article  CAS  Google Scholar 

  4. A. T. Bull, Actinobacteria of the Extremobiosphere, in: Extremophiles, K. Horikosh (ed.), Springer, Japan,Tokyo, 2011, pp. 1203–1240.

  5. P. M. Leung, S. K. Bay, D. V. Meier, E. Chiri, D. A. Cowan, O. Gillor, D. Woebken, and C. Greening, mSystems, 5, e-00495-19 (2020).

  6. S. C. Cary, I. R. McDonald, J. E. Barrett, and D. A. Cowan, Nat. Rev. Microbiol., 8, 129 (2010).

    Article  CAS  Google Scholar 

  7. S. B. Pointing and J. Belnap, Nat. Rev. Microbiol., 10, 551 (2012).

    Article  CAS  Google Scholar 

  8. G. Wang and D. Or, ISME J., 7, 395 (2013).

    Article  CAS  Google Scholar 

  9. T. P. Makhalanyane, A. Valverde, E. Gunnigle, A. Frossard, J. B. Ramond, and D. A. Cowan, FEMS Microbiol. Rev., 39, 203 (2015).

    Article  CAS  Google Scholar 

  10. J. P. Schimel, Annu. Rev. Ecol. Evol. S., 49, 409 (2018).

    Article  Google Scholar 

  11. E. F. DeLong, Nat. Rev. Microbiol., 5, 755 (2007).

    Article  CAS  Google Scholar 

  12. T. K. Mohanta and H. Bae, Biol. Proc. Online, 17, 8 (2015).

    Article  Google Scholar 

  13. S. S. Ebada and W. Ebrahim, Phytochem. Lett., 36, 95 (2020).

    Article  CAS  Google Scholar 

  14. I. Uchida, Y. Itoh, T. Namiki, M. Nishikawa, and M. Hashimoto, Tetrahedron Lett., 27, 2015 (1986).

    Article  CAS  Google Scholar 

  15. T. Namiki, M. Nishikawa, Y. Itoh, I. Uchida, and M. Hashimoto, J. Antibiot., 40, 1400 (1987).

    Article  CAS  Google Scholar 

  16. X. Niu, H.-M. Dahse, K.-D. Menzel, O. Lozach, G. Walther, L. Meijer, S. Grabley, and I. Sattler, J. Nat. Prod., 71, 689 (2008).

    Article  CAS  Google Scholar 

  17. H. Chen, G. Daletos, M. S. Abdel-Aziz, D. Thomy, H. Dai, H. Broetz-Oesterhelt, W. Lin, and P. Proksch, Phytochem. Lett., 12, 35 (2015).

    Article  Google Scholar 

  18. R. R. Parvatkar, C. D’Souza, A. Tripathi, and C. G. Naik, Phytochemistry, 70, 128 (2009).

    Article  CAS  Google Scholar 

  19. T. Namiki, Y. Baba, Y. Suzuki, M. Nishikawa, K. Sawada, Y. Itoh, T. Oku, Y. Kitaura, and M. Hashimoto, Chem. Pharm. Bull., 36, 1404 (1988).

    Article  CAS  Google Scholar 

  20. N. Okamura, H. Haraguchi, K. Hashimoto, and A. Yagi, Phytochemistry, 34, 1005 (1993).

    Article  CAS  Google Scholar 

  21. W.-J. Lan, W. Liu, W.-L. Liang, Z. Xu, X. Le, J. Xu, C.-K. Lam, D.-P. Yang, H.-J. Li, and L.-Y. Wang, Mar. Drugs, 12, 4188 (2014).

    Article  CAS  Google Scholar 

  22. M. M. S. Nagia, M. M. El-Metwally, M. Shaaban, S. M. El-Zalabani, and A. G. Hanna, Org. Med. Chem. Lett., 2, 9 (2012).

    Article  Google Scholar 

  23. R. K. Pettit, Mar. Biotechnol., 13, 1 (2011).

    Article  CAS  Google Scholar 

  24. A. Jaouani, M. Neifar, V. Prigione, A. Ayari, I. Sbissi, S. Ben Amor, S. Ben Tekaya, G. C. Varese, A. Cherif, and M. Gtari, BioMed Res. Int., 2014, 1 (2014).

    Article  Google Scholar 

  25. B. van den Burg, Curr. Opin. Microbiol., 6, 213 (2003).

    Article  Google Scholar 

  26. N. Kostadinova, E. Krumova, S. Tosi, Pashova, and M. Angelova, Biotechnol. Biotechnol. Equip., 23, 267 (2009).

  27. S. S. Ebada and W. Ebrahim, S. Afr. J. Bot., 133 (2020).

  28. Clinical and Laboratory Standards Institute (CLSI), Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts, CLSI Document M27-A3. 3rd Edn., Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, 2008.

  29. Clinical and Laboratory Standards Institute (CLSI), Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically: Approved Standard, 9th Edn., Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, 2012.

  30. W. Ebrahim, F. C. Ozkaya, and S. S. Ebada, S. Afr. J. Bot., 133, 40 (2020).

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Acknowledgment

The authors are gratefully indebted to Dr. Dalia E. El-Badan (Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt) for valuable microbiological discussions in this study.

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Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt

    Weaam Ebrahim

  2. Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, 11566, Egypt

    Sherif S. Ebada

  3. Department of Pharmacognosy, Faculty of Pharmacy, Sinai University, Ismailia, Egypt

    Sherif S. Ebada

Authors
  1. Weaam Ebrahim
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  2. Sherif S. Ebada
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Correspondence to Weaam Ebrahim or Sherif S. Ebada.

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Published in Khimiya Prirodnykh Soedinenii, No. 4, July–August, 2021, pp. 559–562.

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Ebrahim, W., Ebada, S.S. Antimicrobial Metabolites from Extremophilic Fungus Botryotrichum piluliferum Strain WESH19. Chem Nat Compd 57, 654–658 (2021). https://doi.org/10.1007/s10600-021-03443-6

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  • DOI: https://doi.org/10.1007/s10600-021-03443-6

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