Isolation and Characterization of Antimicrobial Peptides from Bos taurus Bone Marrow
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Polypeptides from Bos taurus bone marrow (BBM) were purified for the first time using ultrafiltration and two-stage ion-exchange chromatography. Extract of BBM contained 25 different polypeptides with molecular masses in the range 1027.84–9916.32 Da. The water-soluble fraction of BBM contained large amounts of charged polypeptides. The isolated peptides and proteins possessed dose-dependent antimicrobial activity against pathogenic Candida albicans and Escherichia coli.
Keywordsbovine bone marrow polypeptides ion-exchange chromatography electrophoresis in polyacrylamide gel (PAAG) peptide charge antimicrobial properties
The research was financially supported by the National Research Program of China No. 81660380 “Study of Rehabilitation Care and Its Assessment Criteria for Treating Dysfunction Syndrome by Combining Western and Uyghur Medicines” and “The Thousand Talents Plan of China.” We also thank the Central Asia Drug Research and Development Center of the CAS, Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry of the CAS, and the Academy of Sciences of the Republic of Uzbekistan for sponsoring the work.
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