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Chemistry of Natural Compounds

, Volume 54, Issue 3, pp 523–526 | Cite as

Isolation and Purification of Active Proteases from Sheep Abomasum and Their Biological Activity

  • B. Liu
  • O. Abdullaev
  • O. S. Charyshnikova
  • Sh. Ya. Mirzaakhmedov
  • A. Yili
  • H. A. Aisa
Article
  • 8 Downloads

Sheep abomasum active proteases (SAPs) were purified by chromatography over a column of Sephadex G-25 and by ultrafiltration. The SAPs were found to contain proteases of molecular mass 14 kDa and active peptides with molecular masses varying in the range 651.39–4248.08 Da. Peptide fractions of SAPs reduced significantly (up to 72.0 ± 2.3% inhibition) accumulation of malondialdehyde (MDA) in brain homogenate of laboratory rats.

Keywords

sheep abomasum (SA) peptide extraction and isolation electrophoresis liquid-chromatography–massspectrometry analysis sheep abomasum active proteases (SAPs) proteolytic activity antioxidant activity lipid peroxidation (LPO) malondialdehyde (MDA) 

Notes

Acknowledgment

We thank the Central Asian Drug Research and Development Center of the Chinese Academy of Sciences and the Training Project of Xinjiang Autonomous Region for Technological Innovations of Youth Talents for financial support.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • B. Liu
    • 1
    • 3
  • O. Abdullaev
    • 1
    • 2
  • O. S. Charyshnikova
    • 4
  • Sh. Ya. Mirzaakhmedov
    • 4
  • A. Yili
    • 1
    • 2
  • H. A. Aisa
    • 1
    • 2
  1. 1.Key Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and ChemistryChinese Academy of SciencesUrumqiP. R. China
  2. 2.State Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and ChemistryChinese Academy of SciencesUrumqiP. R. China
  3. 3.University of Chinese Academy of ScienceBeijingP. R. China
  4. 4.Educational and Experimental Center for High TechnologiesTashkentUzbekistan

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