Chemistry of Natural Compounds

, Volume 54, Issue 2, pp 411–414 | Cite as

Heterocyclic Alkaloids as Antimicrobial Agents of Aspergillus fumigatus D Endophytic on Edgeworthia chrysantha

  • Huawei Zhang
  • Chuanfen Ruan
  • Xuelian Bai
  • Jianwei Chen
  • Hong Wang

Growing evidence indicates that endophytes are among the new prolific producers of bioactive natural products [1]. In our screening of antimicrobial endophytes from Edgeworthia chrysantha Lindl., a traditional Chinese medicinal plant used to treat swelling of eye, ophthalmalgia, delacrimation, nephelium of eye, and nocturnal emission [2, 3, 4], the ethyl acetate extract of the fermentation broth of the endophytic strain D exhibited strong antimicrobial activities against pathogenic Escherichia coli, Staphyloccocus aureus, and Candida albicans [5]. A follow-up bioassay-guided fractionation led to the isolation of five heterocyclic alkaloids (1–5) with potent antimicrobial activities.

The mixed metabolic products of the fermentation broth of A. fumigatusD were obtained through liquid-liquid extraction using ethyl acetate and subjected to flash fractionation on a preparative HPLC column to yield six fractions (F0–F5). Antimicrobial assay indicated that fractions F2 and F3 exhibited...



The authors acknowledge financial support from the National Natural Science Foundation of China (Nos. 41776139 and 81773628), the National Key Research and Development Plan (No. 2017YFE0103100), the Zhejiang Natural Science Foundation of China (LY16H300007 and LQ14C200005), and Huahai Student Innovation Program of Zhejiang University of Technology (2015).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Huawei Zhang
    • 1
  • Chuanfen Ruan
    • 1
  • Xuelian Bai
    • 2
  • Jianwei Chen
    • 1
  • Hong Wang
    • 1
  1. 1.School of Pharmaceutical SciencesZhejiang University of TechnologyHangzhouP. R. China
  2. 2.College of Life and Environmental SciencesHangzhou Normal UniversityHangzhouP. R. China

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